Growth suppression induced by antitumor drug vitamin K-3 in human hepatoma HepG2 cells is accompanied by downregulation of proliferating cell nuclear antigen expression

  • Authors:
    • C Juan
    • F Wu
  • View Affiliations

  • Published online on: May 1, 1997     https://doi.org/10.3892/ijo.10.5.1015
  • Pages: 1015-1019
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Abstract

The effects of VK3 on the expression of proliferating cell nuclear antigen (PCNA) and tumor suppresser p53 were investigated in human hepatoma HEPG2 cells. VK3 (50-250 mu M) for 3 h-treatment, induced 2- to 5-fold increase in PCNA mRNA. Cells treated with 100 mu M of VK3 for 0-8 h, showed PCNA mRNA initially increased but subsequently decreased. PCNA protein decreased in 75-100 mu M VK3-treated asynchronous cells but decreased slightly in 100 mu M VK3-treated synchronized cells. p53 protein increased 2-fold in VK3-treated synchronized cells. No changes in PCNA were noted with coincubation of VK3 and actinomycin D, alpha-amanitin, or cycloheximide. This evidence shows that VK3-induced cell growth inhibition and cell cycling events are accompanied by down-regulation of PCNA protein expression.

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May 1997
Volume 10 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Juan C and Juan C: Growth suppression induced by antitumor drug vitamin K-3 in human hepatoma HepG2 cells is accompanied by downregulation of proliferating cell nuclear antigen expression. Int J Oncol 10: 1015-1019, 1997
APA
Juan, C., & Juan, C. (1997). Growth suppression induced by antitumor drug vitamin K-3 in human hepatoma HepG2 cells is accompanied by downregulation of proliferating cell nuclear antigen expression. International Journal of Oncology, 10, 1015-1019. https://doi.org/10.3892/ijo.10.5.1015
MLA
Juan, C., Wu, F."Growth suppression induced by antitumor drug vitamin K-3 in human hepatoma HepG2 cells is accompanied by downregulation of proliferating cell nuclear antigen expression". International Journal of Oncology 10.5 (1997): 1015-1019.
Chicago
Juan, C., Wu, F."Growth suppression induced by antitumor drug vitamin K-3 in human hepatoma HepG2 cells is accompanied by downregulation of proliferating cell nuclear antigen expression". International Journal of Oncology 10, no. 5 (1997): 1015-1019. https://doi.org/10.3892/ijo.10.5.1015