Changes in chromosomes 1 and 6 associated with human cell immortalisation
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- Published online on: May 1, 1997 https://doi.org/10.3892/ijo.10.5.977
- Pages: 977-981
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Abstract
A normal diploid human fibroblast cell line infected with HL60 c-myc has become immortal in vitro following continuous passage. At early passage post-infection the cells exhibited a normal diploid karyotype and in the absence of karyotypic changes grew well beyond the normal passage at which senescence occurred in the normal non-transfected parental cell line or the vector-infected control. Immortalisation of both the infected population and a subclone taken prior to karyotypic change was associated with two independent events involving chromosome 1. The subcloned population also possessed a deletion of the long arm of chromosome 6.