Inhibition of telomerase activity correlates with a decrease in the RNA component of telomerase during differentiation

  • Authors:
    • T Kobayashi
    • K Clodi
    • Y Yang
    • S Ruan
    • H Shiku
    • M Andreeff
    • J Shay
    • W Zhang
  • View Affiliations

  • Published online on: July 1, 1997     https://doi.org/10.3892/ijo.11.1.13
  • Pages: 13-17
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Abstract

In most normal somatic cells the telomeres of human chromosomes shorten with each cell division because of low expression or lack of telomerase activity. Telomerase, a ribonucleoprotein that synthesizes telomeric DNA onto chromosomal ends, is reactivated or upregulated in tumor cells and maintains the stability of telomere length. We previously showed that treatment of HL60 leukemia cells with differentiation-inducing agents resulted in inhibition of telomerase activity. In the present study, we found that the decrease in telomerase activity did not temporally correlate with the expression of a differentiation marker, CD11b, on the cell surface. Mixing of protein extracts from telomerase-negative differentiated HL60 cells with those from parental HL60 cells did not result in inhibition of telomerase activity, suggesting that a diffusible cellular telomerase inhibitor was not produced in the differentiated cells. However, a decrease in telomerase activity correlated with a selective decrease of telomerase RNA expression, a decrease in the levels of total cellular RNA, and an increase in cells at G(0) phase.

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July 1997
Volume 11 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kobayashi T, Clodi K, Yang Y, Ruan S, Shiku H, Andreeff M, Shay J and Zhang W: Inhibition of telomerase activity correlates with a decrease in the RNA component of telomerase during differentiation. Int J Oncol 11: 13-17, 1997
APA
Kobayashi, T., Clodi, K., Yang, Y., Ruan, S., Shiku, H., Andreeff, M. ... Zhang, W. (1997). Inhibition of telomerase activity correlates with a decrease in the RNA component of telomerase during differentiation. International Journal of Oncology, 11, 13-17. https://doi.org/10.3892/ijo.11.1.13
MLA
Kobayashi, T., Clodi, K., Yang, Y., Ruan, S., Shiku, H., Andreeff, M., Shay, J., Zhang, W."Inhibition of telomerase activity correlates with a decrease in the RNA component of telomerase during differentiation". International Journal of Oncology 11.1 (1997): 13-17.
Chicago
Kobayashi, T., Clodi, K., Yang, Y., Ruan, S., Shiku, H., Andreeff, M., Shay, J., Zhang, W."Inhibition of telomerase activity correlates with a decrease in the RNA component of telomerase during differentiation". International Journal of Oncology 11, no. 1 (1997): 13-17. https://doi.org/10.3892/ijo.11.1.13