Adenovirus-mediated p53 gene therapy in nasopharyngeal cancer

  • Authors:
    • Y Zeng
    • N Prabhu
    • R Meng
    • W El-Deiry
  • View Affiliations

  • Published online on: August 1, 1997     https://doi.org/10.3892/ijo.11.2.221
  • Pages: 221-226
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Abstract

EBV-associated nasopharyngeal cancer (NPC) occurs with high frequency in China and is a major cause of morbidity and mortality. To explore the potential use of adenovirus-mediated tumor suppressor p53 gene therapy In NPC, we first examined the in vitro effects of p53 introduced into the NPC cell lines RPMI 2650, Fadu and Detroit 562. p21(WAF1/CIP1) induction by chemotherapy was used as a functional assay which revealed that RPMI 2650 expresses wild-type p53 whereas Fadu and Detroit 562 encode mutant p53. Infection with p53-expressing adenovirus (Ad-p53) induced apoptosis and inhibited cell growth in all three NPC cell lines, regardless of the endogenous p53 status. Adenovirus infectivity was greatest in RPMI 2650 cells, with 100% of the cells expressing beta-galactosidase following Ad-LacZ infection using an MOI of 100, as compared to 20-30% infectivity with the other NPC lines. Using RPMI 2650 cells injected into nude mice, we developed an animal model for nasopharyngeal cancer. Established tumors (0.6-0.8 cm) were injected with 5x10(9) PFU Ad-LacZ, Ad-p53 or PBS in a 100 mu l volume. We found evidence for in vivo expression of beta-galactosidase or p53 and p21 up to two weeks following Ad-LacZ or Ad-p53 virus injection respectively. Objective regression of tumor size was observed at two weeks in 4/6 Ad-p53-treated tumors, but not in Ad-LacZ or PBS-treated tumors. The results provide an animal model for human nasopharyngeal cancer, and indicate a potential use of p53 in its therapy in vivo.

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August 1997
Volume 11 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zeng Y, Prabhu N, Meng R and El-Deiry W: Adenovirus-mediated p53 gene therapy in nasopharyngeal cancer. Int J Oncol 11: 221-226, 1997.
APA
Zeng, Y., Prabhu, N., Meng, R., & El-Deiry, W. (1997). Adenovirus-mediated p53 gene therapy in nasopharyngeal cancer. International Journal of Oncology, 11, 221-226. https://doi.org/10.3892/ijo.11.2.221
MLA
Zeng, Y., Prabhu, N., Meng, R., El-Deiry, W."Adenovirus-mediated p53 gene therapy in nasopharyngeal cancer". International Journal of Oncology 11.2 (1997): 221-226.
Chicago
Zeng, Y., Prabhu, N., Meng, R., El-Deiry, W."Adenovirus-mediated p53 gene therapy in nasopharyngeal cancer". International Journal of Oncology 11, no. 2 (1997): 221-226. https://doi.org/10.3892/ijo.11.2.221