Detection of somatic mutations of the bcl-2 oncogene in B cell lymphomas with the t(14;18)

  • Authors:
    • V Pappa
    • S Wilkes
    • A Norton
    • S Phillips
    • A Rohatiner
    • T Lister
    • B Young
  • View Affiliations

  • Published online on: September 1, 1997     https://doi.org/10.3892/ijo.11.3.481
  • Pages: 481-488
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The incidence of mutations within the first 582 bp of the open reading frame of the bcl-2 gene, has been investigated in presentation lymph node samples, from 7 cases with follicular non-Hodgkin's lymphoma (NHL), 1 case with centroblactic NHL, the DOHH, cell line derived from the immunoblastic transformation of a follicular NHL and one case with benign follicular hyperplasia. A total number of 43 point mutations within the examined portion of the bcl-2 gene were detected in the cases analysed including the DOHH, cell line. Similar analysis of peripheral blood lymphocytes from 2 normal individuals that lacked the t(14;18), revealed no mutations in one case and a single 101 bp A-->G transition in clone, in the other. Missense mutations were detected in 7/8 NHLs, the DOHH2 cell line and the case of benign follicular hyperplasia. There was a significantly higher frequency of mutations within the region corresponding to the BH1, one of the two known functional domains, of the bcl-2 protein. The same position, 445 bp of the bcl-2 gene, was found to be involved in missense mutations affecting the DOHH2 cell line and 3 cases with follicular NHL.

Related Articles

Journal Cover

September 1997
Volume 11 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Pappa V, Wilkes S, Norton A, Phillips S, Rohatiner A, Lister T and Young B: Detection of somatic mutations of the bcl-2 oncogene in B cell lymphomas with the t(14;18). Int J Oncol 11: 481-488, 1997
APA
Pappa, V., Wilkes, S., Norton, A., Phillips, S., Rohatiner, A., Lister, T., & Young, B. (1997). Detection of somatic mutations of the bcl-2 oncogene in B cell lymphomas with the t(14;18). International Journal of Oncology, 11, 481-488. https://doi.org/10.3892/ijo.11.3.481
MLA
Pappa, V., Wilkes, S., Norton, A., Phillips, S., Rohatiner, A., Lister, T., Young, B."Detection of somatic mutations of the bcl-2 oncogene in B cell lymphomas with the t(14;18)". International Journal of Oncology 11.3 (1997): 481-488.
Chicago
Pappa, V., Wilkes, S., Norton, A., Phillips, S., Rohatiner, A., Lister, T., Young, B."Detection of somatic mutations of the bcl-2 oncogene in B cell lymphomas with the t(14;18)". International Journal of Oncology 11, no. 3 (1997): 481-488. https://doi.org/10.3892/ijo.11.3.481