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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 1997 Volume 11 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September 1997 Volume 11 Issue 3

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Article

A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues

  • Authors:
    • T Mancuso
    • S Crovella
    • S Casazza
    • M Pescarolo
    • L Clerico
    • P Russo
    • S Parodi
  • View Affiliations / Copyright

    Affiliations: NATL INST CANC RES,DEPT EXPT ONCOL,I-16132 GENOA,ITALY. UNIV GENOA,DEPT CLIN & EXPT ONCOL,I-16132 GENOA,ITALY. PERKIN ELMER CORP,I-10044 PIANEZZA,TURIN,ITALY. OSPED GALLIERA,I-16128 GENOA,ITALY.
  • Pages: 527-531
    |
    Published online on: September 1, 1997
       https://doi.org/10.3892/ijo.11.3.527
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Abstract

We describe a simple and fast method for the detection and localization of low copy numbers of HPV DNA in formalin-fixed and paraffin-embedded archival tissues. We have developed a protocol for direct bl situ-PCR in order to demonstrate its convenience in rapid and reproducible assessment of HPV infection in unknown biopsies. The morphological aspect of the tissues has been maintained, despite the multiple steps of fixation, permeabilization and thermal cycling, and positivity has been detected only in virus target cells.

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Copy and paste a formatted citation
Spandidos Publications style
Mancuso T, Crovella S, Casazza S, Pescarolo M, Clerico L, Russo P and Parodi S: A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues. Int J Oncol 11: 527-531, 1997.
APA
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., & Parodi, S. (1997). A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues. International Journal of Oncology, 11, 527-531. https://doi.org/10.3892/ijo.11.3.527
MLA
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., Parodi, S."A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues". International Journal of Oncology 11.3 (1997): 527-531.
Chicago
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., Parodi, S."A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues". International Journal of Oncology 11, no. 3 (1997): 527-531. https://doi.org/10.3892/ijo.11.3.527
Copy and paste a formatted citation
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Spandidos Publications style
Mancuso T, Crovella S, Casazza S, Pescarolo M, Clerico L, Russo P and Parodi S: A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues. Int J Oncol 11: 527-531, 1997.
APA
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., & Parodi, S. (1997). A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues. International Journal of Oncology, 11, 527-531. https://doi.org/10.3892/ijo.11.3.527
MLA
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., Parodi, S."A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues". International Journal of Oncology 11.3 (1997): 527-531.
Chicago
Mancuso, T., Crovella, S., Casazza, S., Pescarolo, M., Clerico, L., Russo, P., Parodi, S."A fast and simple in situ PCR method to detect human papilloma virus infection in archival paraffin-embedded tissues". International Journal of Oncology 11, no. 3 (1997): 527-531. https://doi.org/10.3892/ijo.11.3.527
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