Role of ras oncogene in adriamycin resistance in human prostate tumor cells

  • Authors:
    • B Sinha
    • K Song
  • View Affiliations

  • Published online on: October 1, 1997     https://doi.org/10.3892/ijo.11.4.819
  • Pages: 819-823
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Abstract

We have previously isolated a drug-resistant, [PC3(R)], variant of human prostate PC3 cell line, which showed significant resistance (>10-fold) to adriamycin. No known mechanisms of drug resistance were found; however, resistant cells expressed more bcl2, c-myc, and ms oncogenes compared to the sensitive cells. In this study, we found that buthionine sulfoximine (BSO), an inhibitor of gamma-glutamyl-cysteine synthetase, decreased glutathione levels by 80-90% in both cell lines. BSO treatment down-modulated Ras protein only in PC3(R) cells and caused a 4-fold sensitization of PC3(R) cells to adriamycin without affecting PC3(W) cells. Farnesol treatment also inhibited expression of Ras protein and concomitantly reversed adriamycin resistance in PC3(R) cells, indicating that altered levels of ras expression plays an important role in drug resistance in PC3(R) cells.

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October 1997
Volume 11 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sinha B and Sinha B: Role of ras oncogene in adriamycin resistance in human prostate tumor cells. Int J Oncol 11: 819-823, 1997
APA
Sinha, B., & Sinha, B. (1997). Role of ras oncogene in adriamycin resistance in human prostate tumor cells. International Journal of Oncology, 11, 819-823. https://doi.org/10.3892/ijo.11.4.819
MLA
Sinha, B., Song, K."Role of ras oncogene in adriamycin resistance in human prostate tumor cells". International Journal of Oncology 11.4 (1997): 819-823.
Chicago
Sinha, B., Song, K."Role of ras oncogene in adriamycin resistance in human prostate tumor cells". International Journal of Oncology 11, no. 4 (1997): 819-823. https://doi.org/10.3892/ijo.11.4.819