Expression of mRNA for luteinizing hormone-releasing hormone receptors and epidermal growth factor receptors in human cancer cell lines.

  • Authors:
    • N Lamharzi
    • G Halmos
    • P Armatis
    • A V Schally
  • View Affiliations

  • Published online on: March 1, 1998     https://doi.org/10.3892/ijo.12.3.671
  • Pages: 671-676
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Abstract

Conventional chemotherapy produces varying degrees of response in patients with many advanced cancers and has significant side effects. Receptors for luteinizing hormone-releasing hormone (LH-RH) are present in a high percentage of human ovarian, prostatic, breast and endometrial tumors and targeted chemotherapy based on cytotoxic analogs of LH-RH might yield better results. The present study was undertaken to determine whether human cancer cell lines express mRNA for LH-RH receptors and epidermal growth factor (EGF) receptors. Using radioligand binding studies, we showed the presence of high-affinity binding sites for LH-RH and EGF in the membranes of human ovarian, prostatic, breast and endometrial cancer cell lines as well as in the JAR choriocarcinoma cell line. The expression of the mRNA for LH-RH receptors and EGF receptors in these cell lines was demonstrated by RT-PCR using specific primers and by subsequent Southern blot analysis. The PCR products obtained were of the expected size, 319 bp for LH-RH receptors and 400 bp for EGF receptors. These findings support the view that cytotoxic analogs of LH-RH could be used for targeted chemotherapy of these cancers. Moreover, the results suggest that these human cancer cell lines might have local regulatory systems for their proliferation based on LH-RH and EGF. Further investigations are required to elucidate the signal transduction pathways involved in the effects of cytotoxic LH-RH analogs on human tumors.

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Mar 1998
Volume 12 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lamharzi N, Halmos G, Armatis P and Schally A: Expression of mRNA for luteinizing hormone-releasing hormone receptors and epidermal growth factor receptors in human cancer cell lines.. Int J Oncol 12: 671-676, 1998
APA
Lamharzi, N., Halmos, G., Armatis, P., & Schally, A. (1998). Expression of mRNA for luteinizing hormone-releasing hormone receptors and epidermal growth factor receptors in human cancer cell lines.. International Journal of Oncology, 12, 671-676. https://doi.org/10.3892/ijo.12.3.671
MLA
Lamharzi, N., Halmos, G., Armatis, P., Schally, A."Expression of mRNA for luteinizing hormone-releasing hormone receptors and epidermal growth factor receptors in human cancer cell lines.". International Journal of Oncology 12.3 (1998): 671-676.
Chicago
Lamharzi, N., Halmos, G., Armatis, P., Schally, A."Expression of mRNA for luteinizing hormone-releasing hormone receptors and epidermal growth factor receptors in human cancer cell lines.". International Journal of Oncology 12, no. 3 (1998): 671-676. https://doi.org/10.3892/ijo.12.3.671