Usefulness of K-ras gene mutation at codon 12 in bile for diagnosing biliary strictures.

  • Authors:
    • R Ito
    • K Tamura
    • H Ashida
    • M Nishiwaki
    • A Nishioka
    • Y Yamamoto
    • J I Furuyama
    • J Utsunomiya
  • View Affiliations

  • Published online on: May 1, 1998     https://doi.org/10.3892/ijo.12.5.1019
  • Pages: 1019-1042
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Abstract

Point mutations of the K-ras gene at codon 12 are often detected in the pancreatic juice of patients with pancreatic cancer. Detection of these mutations may, thus, have diagnostic implications. K-ras mutations may also have diagnostic potential for other biliary tumors. We sought to detect K-ras mutations in DNA obtained from bile in patients with biliary tract cancers, pancreatic cancer and benign biliary disease but who had obstructive jaundice. In 35 patients, bile was collected during percutaneous transhepatic choledocal drainage (PTCD) catheters. K-ras gene mutations at codon 12 in the samples were examined using mutant-allele-specific-amplification (MASA). We compared these results with cytological analyses of bile. K-ras mutations at codon 12 in bile were detected in 11 of 14 (79%) of the patients with biliary duct cancer, 3 of 9 (33%) with pancreatic cancer but not in patients with gallbladder cancer (n=3), papilla of Vater's cancer (n=3) or benign biliary diseases (n=6). In the patients, where cytological evaluation did not reveal malignant cells, K-ras mutations in bile were detected in 5 of 7 (71%) patients with biliary duct cancer and 2 of 5 (40%) with pancreatic cancer. This approach, when used in conjunction with bile cytology, may improve the yield in diagnosing suspected malignant tumors of the pancreatic-biliary system.

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May 1998
Volume 12 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ito R, Tamura K, Ashida H, Nishiwaki M, Nishioka A, Yamamoto Y, Furuyama J and Utsunomiya J: Usefulness of K-ras gene mutation at codon 12 in bile for diagnosing biliary strictures.. Int J Oncol 12: 1019-1042, 1998
APA
Ito, R., Tamura, K., Ashida, H., Nishiwaki, M., Nishioka, A., Yamamoto, Y. ... Utsunomiya, J. (1998). Usefulness of K-ras gene mutation at codon 12 in bile for diagnosing biliary strictures.. International Journal of Oncology, 12, 1019-1042. https://doi.org/10.3892/ijo.12.5.1019
MLA
Ito, R., Tamura, K., Ashida, H., Nishiwaki, M., Nishioka, A., Yamamoto, Y., Furuyama, J., Utsunomiya, J."Usefulness of K-ras gene mutation at codon 12 in bile for diagnosing biliary strictures.". International Journal of Oncology 12.5 (1998): 1019-1042.
Chicago
Ito, R., Tamura, K., Ashida, H., Nishiwaki, M., Nishioka, A., Yamamoto, Y., Furuyama, J., Utsunomiya, J."Usefulness of K-ras gene mutation at codon 12 in bile for diagnosing biliary strictures.". International Journal of Oncology 12, no. 5 (1998): 1019-1042. https://doi.org/10.3892/ijo.12.5.1019