Mutation of the transforming growth factor-beta type II receptor gene is a rare event in human sporadic gastric carcinomas.

  • Authors:
    • Y Shitara
    • H Yokozaki
    • W Yasui
    • S Takenoshita
    • Y Nagamachi
    • E Tahara
  • View Affiliations

  • Published online on: May 1, 1998     https://doi.org/10.3892/ijo.12.5.1061
  • Pages: 1061-1066
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Abstract

Mutations of the transforming growth factor-beta type II receptor (TGF-beta RII) gene have been detected in several human cancers. However, mutation analysis of coding sequences of TGF-beta RII in gastric carcinomas has not yet been fully elucidated. We performed PCR-SSCP analysis and direct DNA sequencing of the entire coding region of TGF- RII in 38 human sporadic gastric cancers and 8 gastric cancer cell lines. Mutations of the TGF-beta RII were detected in two tumors and three cell lines. Two tumors had one base deletion in the polyadenine tract in exon 3, the cystein-rich extracellular domain. Three cell lines had a silent mutation in the kinase domain located in exon 4. Polymorphisms were detected in introns 2 and 3. An a/g polymorphism was observed at the seventh base in intron 2 and an a/t polymorphism was observed at the fourth to last base in intron 3. There were no mutations in exons 1, 2, 5, 6 and 7. These results indicate that the polyadenine tract in the TGF-beta RII is a mutational hot spot in human gastric cancer. However, these results also suggest that mutations of the gene are rare events in human sporadic gastric cancer.

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May 1998
Volume 12 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Shitara Y, Yokozaki H, Yasui W, Takenoshita S, Nagamachi Y and Tahara E: Mutation of the transforming growth factor-beta type II receptor gene is a rare event in human sporadic gastric carcinomas.. Int J Oncol 12: 1061-1066, 1998.
APA
Shitara, Y., Yokozaki, H., Yasui, W., Takenoshita, S., Nagamachi, Y., & Tahara, E. (1998). Mutation of the transforming growth factor-beta type II receptor gene is a rare event in human sporadic gastric carcinomas.. International Journal of Oncology, 12, 1061-1066. https://doi.org/10.3892/ijo.12.5.1061
MLA
Shitara, Y., Yokozaki, H., Yasui, W., Takenoshita, S., Nagamachi, Y., Tahara, E."Mutation of the transforming growth factor-beta type II receptor gene is a rare event in human sporadic gastric carcinomas.". International Journal of Oncology 12.5 (1998): 1061-1066.
Chicago
Shitara, Y., Yokozaki, H., Yasui, W., Takenoshita, S., Nagamachi, Y., Tahara, E."Mutation of the transforming growth factor-beta type II receptor gene is a rare event in human sporadic gastric carcinomas.". International Journal of Oncology 12, no. 5 (1998): 1061-1066. https://doi.org/10.3892/ijo.12.5.1061