Loss of merlin-p85 protein complex in NF2-related tumors.

Takeshima,H; Nishi,T; Yamamoto,K; Kino,T; Nakamura,H; Saya,H; Kochi,M; Kuratsu,J I; Ushio,Y

doi:10.3892/ijo.12.5.1073

Loss of merlin-p85 protein complex in NF2-related tumors.

Authors:
- H Takeshima
- T Nishi
- K Yamamoto
- T Kino
- H Nakamura
- H Saya
- M Kochi
- J I Kuratsu
- Y Ushio
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Affiliations: Department of Neurosurgery, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto, 860, Japan.
Published online on: May 1, 1998 https://doi.org/10.3892/ijo.12.5.1073
Pages: 1073-1081

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Abstract

The NF2 tumor suppressor gene product, designated merlin, belongs to the family of molecules that links membranous protein with the cytoskeleton. We have previously shown that merlin was co-immunoprecipitated with a cellular protein, p85, in cultured cell. To analyze the alteration of merlin and associated proteins in surgical specimens, we developed a new method for biotin-labeling of whole cellular proteins. Screening of tumor tissues using our method showed that none of malignant gliomas and half of the NF2-related tumors had altered p85 and merlin. Our detection method seems useful for the screening of merlin alterations in NF2-related tumors.