Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.

  • Authors:
    • T Aikoh
    • A Tomokuni
    • T Matsukii
    • F Hyodoh
    • H Ueki
    • T Otsuki
    • A Ueki
  • View Affiliations

  • Published online on: June 1, 1998     https://doi.org/10.3892/ijo.12.6.1355
  • Pages: 1355-1364
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Abstract

Silica and related substances such as silicate have been proven to possess "adjuvant effects". We have previously reported a finding of polyclonal human T cell activation induced by silicate as a superantigen in vitro. In this study, we observed activation-induced cell death in human lymphocytes after stimulation with chrysotile, a kind of silicate. Apoptotic cells were detected flow cytometrically using the TUNEL assay, and the maximum appearance of TUNEL positive cells occurred on day 4 of incubation. Simultaneously the manifestation of small-sized cells in the specimens increased implying apoptosis. Fas expression on lymphocytes increased to day 3 of incubation with chrysotile, and then spontaneously decreased on day 4 when remarkable apoptosis could be detected. Based on these results it is conceivable that activation-induced cell death occurred through Fas-Fas ligand interaction in lymphocytes after stimulation with silicate in a concentration with which no acute cytotoxicity has been detected. Whether and how the repeated apoptosis in definite clones of lymphocytes causes the induction of sFas synthesis need clarification.

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Jun 1998
Volume 12 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Aikoh T, Tomokuni A, Matsukii T, Hyodoh F, Ueki H, Otsuki T and Ueki A: Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.. Int J Oncol 12: 1355-1364, 1998
APA
Aikoh, T., Tomokuni, A., Matsukii, T., Hyodoh, F., Ueki, H., Otsuki, T., & Ueki, A. (1998). Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.. International Journal of Oncology, 12, 1355-1364. https://doi.org/10.3892/ijo.12.6.1355
MLA
Aikoh, T., Tomokuni, A., Matsukii, T., Hyodoh, F., Ueki, H., Otsuki, T., Ueki, A."Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.". International Journal of Oncology 12.6 (1998): 1355-1364.
Chicago
Aikoh, T., Tomokuni, A., Matsukii, T., Hyodoh, F., Ueki, H., Otsuki, T., Ueki, A."Activation-induced cell death in human peripheral blood lymphocytes after stimulation with silicate in vitro.". International Journal of Oncology 12, no. 6 (1998): 1355-1364. https://doi.org/10.3892/ijo.12.6.1355