Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.

  • Authors:
    • L J Lipinski
    • H L Ross
    • B Zajc
    • J M Sayer
    • D M Jerina
    • A Dipple
  • View Affiliations

  • Published online on: August 1, 1998     https://doi.org/10.3892/ijo.13.2.269
  • Pages: 269-342
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Abstract

Neither Sequenase 2.0 nor Klenow fragment were able to extend 12-mer primers using the eight templates (16-mers) derived by placing each of the four isomeric benzo[a]pyrene diol epoxide-deoxyguanosine adducts at the 13th nucleotide from the 3'-end of two different sequence contexts. Using an 11-mer primer to get a running start did not overcome the adduct induced block of primer extension except for the Klenow fragment and one of the two sequence contexts, indicating primer extension is dependent on both the polymerase and sequence context. In this case, purine nucleoside triphosphates (dATP>dGTP) were incorporated opposite each of the four adducts.

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Aug 1998
Volume 13 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lipinski L, Ross H, Zajc B, Sayer J, Jerina D and Dipple A: Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.. Int J Oncol 13: 269-342, 1998.
APA
Lipinski, L., Ross, H., Zajc, B., Sayer, J., Jerina, D., & Dipple, A. (1998). Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.. International Journal of Oncology, 13, 269-342. https://doi.org/10.3892/ijo.13.2.269
MLA
Lipinski, L., Ross, H., Zajc, B., Sayer, J., Jerina, D., Dipple, A."Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.". International Journal of Oncology 13.2 (1998): 269-342.
Chicago
Lipinski, L., Ross, H., Zajc, B., Sayer, J., Jerina, D., Dipple, A."Effect of single benzo[a]pyrene diol epoxide-deoxyguanosine adducts on the action of DNA polymerases in vitro.". International Journal of Oncology 13, no. 2 (1998): 269-342. https://doi.org/10.3892/ijo.13.2.269