Inhibitory effects of an antiestrogen, toremifene, on the phorbol ester-induced adhesive capacity of breast carcinoma cells.

  • Authors:
    • M Maemura
    • Y Iino
    • J Horiguchi
    • H Takei
    • Y Horii
    • Y Koibuchi
    • T Yokoe
    • I Takeyoshi
    • S Ohwada
    • Y Morishita
  • View Affiliations

  • Published online on: November 1, 1998     https://doi.org/10.3892/ijo.13.5.981
  • Pages: 981-986
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Abstract

Previous studies have revealed that protein kinase C (PKC) is responsible for malignant progression. In the present study, we investigated the potent inhibitory effects of an antiestrogen, toremifene, on PKC-mediated cellular adhesion. A phorbol ester, phorbol 12-myristate 13-acetate (PMA), significantly enhanced alpha2beta1 integrin-dependent adhesion of MCF-7 breast carcinoma cells. This PMA-induced adhesion was partially inhibited by incubating cells with toremifene prior to PMA exposure in a time- and dose-dependent manner. FACS analysis demonstrated that the PMA-induced alpha2beta1-dependent cellular adhesion was accompanied with elevated expression of alpha2beta1+integrin subunit on the cell surface. However, toremifene did not affect the elevated expression levels of these integrins but rather the avidity of alpha2beta1 integrin. We concluded that toremifene inhibited cellular adhesion activated by PMA, probably through mechanism which inhibits PKC.

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Nov 1998
Volume 13 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Maemura M, Iino Y, Horiguchi J, Takei H, Horii Y, Koibuchi Y, Yokoe T, Takeyoshi I, Ohwada S, Morishita Y, Morishita Y, et al: Inhibitory effects of an antiestrogen, toremifene, on the phorbol ester-induced adhesive capacity of breast carcinoma cells.. Int J Oncol 13: 981-986, 1998.
APA
Maemura, M., Iino, Y., Horiguchi, J., Takei, H., Horii, Y., Koibuchi, Y. ... Morishita, Y. (1998). Inhibitory effects of an antiestrogen, toremifene, on the phorbol ester-induced adhesive capacity of breast carcinoma cells.. International Journal of Oncology, 13, 981-986. https://doi.org/10.3892/ijo.13.5.981
MLA
Maemura, M., Iino, Y., Horiguchi, J., Takei, H., Horii, Y., Koibuchi, Y., Yokoe, T., Takeyoshi, I., Ohwada, S., Morishita, Y."Inhibitory effects of an antiestrogen, toremifene, on the phorbol ester-induced adhesive capacity of breast carcinoma cells.". International Journal of Oncology 13.5 (1998): 981-986.
Chicago
Maemura, M., Iino, Y., Horiguchi, J., Takei, H., Horii, Y., Koibuchi, Y., Yokoe, T., Takeyoshi, I., Ohwada, S., Morishita, Y."Inhibitory effects of an antiestrogen, toremifene, on the phorbol ester-induced adhesive capacity of breast carcinoma cells.". International Journal of Oncology 13, no. 5 (1998): 981-986. https://doi.org/10.3892/ijo.13.5.981