Increased invasion of neuroglioma cells transfected with urokinase plasminogen activator receptor cDNA.

  • Authors:
    • S Mohanam
    • S K Chintala
    • P M Mohan
    • R Sawaya
    • G K Lagos
    • Z L Gokaslan
    • G P Kouraklis
    • J S Rao
  • View Affiliations

  • Published online on: December 1, 1998     https://doi.org/10.3892/ijo.13.6.1285
  • Pages: 1285-1375
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Abstract

The cell-surface urokinase plasminogen activator receptor (uPAR) plays a key role in regulating plasminogen cleavage during extracellular proteolysis. Our recent results demonstrated that uPAR expression is critical for the invasiveness of human gliomas and down regulation of uPAR caused by antisense cDNA transfection inhibits the invasion of these stable antisense uPAR-transfectant clones. To study the role of uPARs in glioma cell invasion, a human neuroglioma cell line (H4) that normally produces low numbers of uPARs was transfected with the expression vector containing full-length human uPAR cDNA. Stable transfectants were analyzed for uPAR mRNA expression, receptor number, in vitro invasion and secretion of uPA and MMP-2. The uPAR-overproducing clones showed a 4-fold increase in uPAR mRNA transcription and approximately 40% increase in receptor numbers. uPAR-overproducing clones also invaded through matrigel to a significantly greater extent than did parent cell line and vector clones. However, the uPAR-overexpressing clones and parent cell lines showed similar uPA and MMP-2 activities. These results suggest that the over-production of uPAR on the surface of neuroglioma cells enhances the invasiveness.

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Dec 1998
Volume 13 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Mohanam S, Chintala S, Mohan P, Sawaya R, Lagos G, Gokaslan Z, Kouraklis G and Rao J: Increased invasion of neuroglioma cells transfected with urokinase plasminogen activator receptor cDNA.. Int J Oncol 13: 1285-1375, 1998
APA
Mohanam, S., Chintala, S., Mohan, P., Sawaya, R., Lagos, G., Gokaslan, Z. ... Rao, J. (1998). Increased invasion of neuroglioma cells transfected with urokinase plasminogen activator receptor cDNA.. International Journal of Oncology, 13, 1285-1375. https://doi.org/10.3892/ijo.13.6.1285
MLA
Mohanam, S., Chintala, S., Mohan, P., Sawaya, R., Lagos, G., Gokaslan, Z., Kouraklis, G., Rao, J."Increased invasion of neuroglioma cells transfected with urokinase plasminogen activator receptor cDNA.". International Journal of Oncology 13.6 (1998): 1285-1375.
Chicago
Mohanam, S., Chintala, S., Mohan, P., Sawaya, R., Lagos, G., Gokaslan, Z., Kouraklis, G., Rao, J."Increased invasion of neuroglioma cells transfected with urokinase plasminogen activator receptor cDNA.". International Journal of Oncology 13, no. 6 (1998): 1285-1375. https://doi.org/10.3892/ijo.13.6.1285