Tributyrin induces growth inhibitory and differentiating effects on HT-29 colon cancer cells in vitro.

Schröder,C; Eckert,K; Maurer,H R

doi:10.3892/ijo.13.6.1335

Dec 1998 Volume 13 Issue 6

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Dec 1998 Volume 13 Issue 6

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Article

Tributyrin induces growth inhibitory and differentiating effects on HT-29 colon cancer cells in vitro.

Authors:
- C Schröder
- K Eckert
- H R Maurer
View Affiliations / Copyright

Affiliations: Institut fur Pharmazie der Freien Universitat Berlin, Germany.
Pages: 1335-1375
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Published online on: December 1, 1998

https://doi.org/10.3892/ijo.13.6.1335
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Abstract

Tributyrin (TB) is a prodrug of butyrate known to induce tumor cells to differentiate. We examined its effects on cell growth, viability, cellular morphology and differentiation of HT-29 colon cancer cells in vitro, as reflected by the expression of CEA, E-cadherin and the induction of alkaline phosphatase activity. TB, applied in a stable emulsion, inhibited tumor cell proliferation in a reversible and dose-dependent manner (0.5-4 mM) with significant morphological changes. The IC50 value of TB was 1 mM after 6 days. For comparison, sodium butyrate, applied in equimolar concentration, inhibited cell growth with an IC50 value of 2.2 mM. TB treatment at concentrations of 0.5 mM and 2 mM resulted in an increase of the doubling times by 18% and 160%, respectively, without any effects on cell viability. By a colorimetric immunoassay, 1.5 mM TB induced the expression of both CEA and E-cadherin by about 260% and 100%, respectively. Furthermore, the activity of the brush border enzyme alkaline phosphatase was enhanced in a dose-dependent manner, up to 60-fold at the maximum of 2 mM TB. Our results show that TB is more active than butyrate in suppressing cell growth and concomitantly promoting differentiation of HT-29 colon cancer cells. Hence it may be a promising candidate for clinical therapeutic protocols and merits further investigation.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Tributyrin induces growth inhibitory and differentiating effects on HT-29 colon cancer cells in vitro.

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