Deletion monitoring in skin tumors by interphase-FISH using band-specific DNA probes.

  • Authors:
    • M Dobler
    • J Schuh
    • F Kiesewetter
    • H Schell
    • T Liehr
    • E Gebhart
  • View Affiliations

  • Published online on: March 1, 1999     https://doi.org/10.3892/ijo.14.3.571
  • Pages: 571-577
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Abstract

Thin sections from archival paraffin blocks of various skin tumors (26 melanomas, 15 squamous cell carcinomas, 5 keratoacanthomas, 5 basal cell carcinomas) were subjected to interphase-FISH (I-FISH) with DNA probes which are specific for chromosomal regions often involved in deletions in human cancer. These were probes for chromosome 3p21, the p53 gene on chromosome 17p13, and, in a few selected cases, a probe for chromosome 9p21. It was demonstrated that deletions of these regions could be reliably detected and related to tumor type and histology, i.e. grading. The most common deletion was that of 3p21 which was found in all studied squamous cell carcinomas (SCC) of low differentiation, in 60% of the Bowen carcinomas, in 70% of the metastatic melanomas less than 1.5 mm thick, and in over 55% of those which thickness over 2 mm. In contrast, FISH-detected p53 deletion was a rare finding in the investigated tumors. However, this gene was even found in an increased copy number in 60% of the poorly differentiated SCCs (grade 4) and in 50% of the non-metastatic melanomas less than 1.5 mm thick. Deletion of 9p21 was detected in 13 of the 14 tumors on which pertinent examinations could be performed. I-FISH was shown to be a reliable technique for the rapid detection of chromosome band specific deletions in archival material of human skin tumors.

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Mar 1999
Volume 14 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Dobler M, Schuh J, Kiesewetter F, Schell H, Liehr T and Gebhart E: Deletion monitoring in skin tumors by interphase-FISH using band-specific DNA probes.. Int J Oncol 14: 571-577, 1999
APA
Dobler, M., Schuh, J., Kiesewetter, F., Schell, H., Liehr, T., & Gebhart, E. (1999). Deletion monitoring in skin tumors by interphase-FISH using band-specific DNA probes.. International Journal of Oncology, 14, 571-577. https://doi.org/10.3892/ijo.14.3.571
MLA
Dobler, M., Schuh, J., Kiesewetter, F., Schell, H., Liehr, T., Gebhart, E."Deletion monitoring in skin tumors by interphase-FISH using band-specific DNA probes.". International Journal of Oncology 14.3 (1999): 571-577.
Chicago
Dobler, M., Schuh, J., Kiesewetter, F., Schell, H., Liehr, T., Gebhart, E."Deletion monitoring in skin tumors by interphase-FISH using band-specific DNA probes.". International Journal of Oncology 14, no. 3 (1999): 571-577. https://doi.org/10.3892/ijo.14.3.571