Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway.

  • Authors:
    • M Serbulea
    • S Kakumu
    • A A Thant
    • K Miyazaki
    • K Machida
    • T Senga
    • S Ohta
    • K Yoshioka
    • N Hotta
    • M Hamaguchi
  • View Affiliations

  • Published online on: April 1, 1999     https://doi.org/10.3892/ijo.14.4.733
  • Pages: 733-741
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Abstract

Hyaluronan (HA) triggers a wide variety of cellular functions, yet its signaling pathway remains largely unclear. We found that HA-treatment of 3Y1 cells activated tyrosine phosphorylation of cellular proteins and mitogen-activated protein (MAP) kinase in a time- and dose-dependent manner, and, subsequently, stimulated cell growth. This HA-activity was resistant to boiling at 100 degrees C but completely abolished by treatment with hyaluronidase, suggesting that HA itself, but not any HA-associated proteins, has the activity. In addition, we found that HA-dependent activation of MAP kinase was strongly suppressed by the expression of dominant negative ras (S17N ras). These results suggest that Ras-MAP kinase pathway is activated by HA and may play an important role in HA-dependent signaling.

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Apr 1999
Volume 14 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Serbulea M, Kakumu S, Thant A, Miyazaki K, Machida K, Senga T, Ohta S, Yoshioka K, Hotta N, Hamaguchi M, Hamaguchi M, et al: Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway.. Int J Oncol 14: 733-741, 1999
APA
Serbulea, M., Kakumu, S., Thant, A., Miyazaki, K., Machida, K., Senga, T. ... Hamaguchi, M. (1999). Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway.. International Journal of Oncology, 14, 733-741. https://doi.org/10.3892/ijo.14.4.733
MLA
Serbulea, M., Kakumu, S., Thant, A., Miyazaki, K., Machida, K., Senga, T., Ohta, S., Yoshioka, K., Hotta, N., Hamaguchi, M."Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway.". International Journal of Oncology 14.4 (1999): 733-741.
Chicago
Serbulea, M., Kakumu, S., Thant, A., Miyazaki, K., Machida, K., Senga, T., Ohta, S., Yoshioka, K., Hotta, N., Hamaguchi, M."Hyaluronan activates mitogen-activated protein kinase via Ras-signaling pathway.". International Journal of Oncology 14, no. 4 (1999): 733-741. https://doi.org/10.3892/ijo.14.4.733