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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Jun 1999 Volume 14 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Jun 1999 Volume 14 Issue 6

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Article

Expression of G1 phase regulators in MG-63 osteosarcoma cell line.

  • Authors:
    • M Merli
    • M S Benassi
    • G Gamberi
    • P Ragazzini
    • M R Sollazzo
    • L Molendini
    • G Magagnoli
    • C Ferrari
    • M C Maltarello
    • P Picci

  • View Affiliations / Copyright

    Affiliations: Laboratory of Oncologic Research, Rizzoli Orthopaedic Institute, 40136 Bologna, Italy.
  • Pages: 1117-1138
    |
    Published online on: June 1, 1999
       https://doi.org/10.3892/ijo.14.6.1117
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Abstract

Cyclins and cyclin-dependent kinases (cdks) form complexes that govern transitions during cell cycle phases. In this study we characterized a human osteosarcoma cell line, MG-63, for the expression level of cyclin D1, cyclin E, cdk4, cdk2, and cell cycle inhibitors pRb and p21. To investigate the role of these proteins we treated MG-63 cells with tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Cell proliferation analysis demonstrated an increased proliferation of MG-63 cells with IL-6, while TNF-alpha acted as an anti-proliferative agent. Immunoblotting revealed an increased expression of p21 with TNF-alpha and its complex with cdk2. TNF-alpha reduced the expression of the cyclin E-cdk2 complex. TNF-alpha did not affect the amount of cyclin D1, cyclin E, cdk4, cdk2, and of cyclin D1-cdk4 complex. IL-6 decreased p21 expression and its complex with cdk2, while it increased the cyclin E-cdk2 complex. Cyclin D1 and cdk4 expression and their complex did not change after IL-6 treatment, nor did cyclin E and cdk2 protein expression. Hyperphosphorylated/dephosphorylated Rb protein ratio was reduced with TNF-alpha whereas it increased with IL-6. These results may suggest an important role of p21 and of cyclin E-cdk2 complex in the G1 phase regulation through pRb phosphorylation in MG-63 cells.

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Copy and paste a formatted citation
Spandidos Publications style
Merli M, Benassi M, Gamberi G, Ragazzini P, Sollazzo M, Molendini L, Magagnoli G, Ferrari C, Maltarello M, Picci P, Picci P, et al: Expression of G1 phase regulators in MG-63 osteosarcoma cell line.. Int J Oncol 14: 1117-1138, 1999.
APA
Merli, M., Benassi, M., Gamberi, G., Ragazzini, P., Sollazzo, M., Molendini, L. ... Picci, P. (1999). Expression of G1 phase regulators in MG-63 osteosarcoma cell line.. International Journal of Oncology, 14, 1117-1138. https://doi.org/10.3892/ijo.14.6.1117
MLA
Merli, M., Benassi, M., Gamberi, G., Ragazzini, P., Sollazzo, M., Molendini, L., Magagnoli, G., Ferrari, C., Maltarello, M., Picci, P."Expression of G1 phase regulators in MG-63 osteosarcoma cell line.". International Journal of Oncology 14.6 (1999): 1117-1138.
Chicago
Merli, M., Benassi, M., Gamberi, G., Ragazzini, P., Sollazzo, M., Molendini, L., Magagnoli, G., Ferrari, C., Maltarello, M., Picci, P."Expression of G1 phase regulators in MG-63 osteosarcoma cell line.". International Journal of Oncology 14, no. 6 (1999): 1117-1138. https://doi.org/10.3892/ijo.14.6.1117
Copy and paste a formatted citation
x
Spandidos Publications style
Merli M, Benassi M, Gamberi G, Ragazzini P, Sollazzo M, Molendini L, Magagnoli G, Ferrari C, Maltarello M, Picci P, Picci P, et al: Expression of G1 phase regulators in MG-63 osteosarcoma cell line.. Int J Oncol 14: 1117-1138, 1999.
APA
Merli, M., Benassi, M., Gamberi, G., Ragazzini, P., Sollazzo, M., Molendini, L. ... Picci, P. (1999). Expression of G1 phase regulators in MG-63 osteosarcoma cell line.. International Journal of Oncology, 14, 1117-1138. https://doi.org/10.3892/ijo.14.6.1117
MLA
Merli, M., Benassi, M., Gamberi, G., Ragazzini, P., Sollazzo, M., Molendini, L., Magagnoli, G., Ferrari, C., Maltarello, M., Picci, P."Expression of G1 phase regulators in MG-63 osteosarcoma cell line.". International Journal of Oncology 14.6 (1999): 1117-1138.
Chicago
Merli, M., Benassi, M., Gamberi, G., Ragazzini, P., Sollazzo, M., Molendini, L., Magagnoli, G., Ferrari, C., Maltarello, M., Picci, P."Expression of G1 phase regulators in MG-63 osteosarcoma cell line.". International Journal of Oncology 14, no. 6 (1999): 1117-1138. https://doi.org/10.3892/ijo.14.6.1117
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