Suppression of tumorigenicity in cervical carcinoma HeLa cells by an episomal form of adeno-associated virus.

  • Authors:
    • F Y Wu
    • C Y Wu
    • C H Lin
    • C H Wu
  • View Affiliations

  • Published online on: July 1, 1999     https://doi.org/10.3892/ijo.15.1.101
  • Pages: 101-107
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Abstract

To compare the oncosuppressive activity of integrated and episomal adeno-associated virus (AAV), two Epstein-Barr virus (EBV) derived episomal form vectors, p205 and p220, were used to generate plasmids containing episomal AAV. HeLa cells transfected with p205, p220, and the plasmids with forward (pA205-1, pA220-1) and reverse (pA205-1, pA220-2) orientation of inserted AAV DNA were designated H205, H220, HA205-1, HA220-1, HA205-1, and Ha220-2, respectively. The respective average copy numbers of pA205 and pA220 per cell are 4.9-6.9 and 4.3. The AAV-transfected HeLa cells displayed growth inhibition when compared to parental and the vector-transfected HeLa cells. Nude mice assay showed that the tumor size from HA205-1 and HA205-2 cells were 11.3 and 22.6%, and those from HA220-1 and HA220-2 cells were 54.8 and 57.3%, respectively, when compared to parental HeLa cells. HA205-1 cells containing forward AAV insert exerted more oncosuppressive effect than HA205-2 cells containing reverse AAV insert. Our data indicate that the episomal AAV can exert oncosuppressive activity as compared to the integrated AAV in HeLa cells.

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Jul 1999
Volume 15 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wu F, Wu C, Lin C and Wu C: Suppression of tumorigenicity in cervical carcinoma HeLa cells by an episomal form of adeno-associated virus.. Int J Oncol 15: 101-107, 1999
APA
Wu, F., Wu, C., Lin, C., & Wu, C. (1999). Suppression of tumorigenicity in cervical carcinoma HeLa cells by an episomal form of adeno-associated virus.. International Journal of Oncology, 15, 101-107. https://doi.org/10.3892/ijo.15.1.101
MLA
Wu, F., Wu, C., Lin, C., Wu, C."Suppression of tumorigenicity in cervical carcinoma HeLa cells by an episomal form of adeno-associated virus.". International Journal of Oncology 15.1 (1999): 101-107.
Chicago
Wu, F., Wu, C., Lin, C., Wu, C."Suppression of tumorigenicity in cervical carcinoma HeLa cells by an episomal form of adeno-associated virus.". International Journal of Oncology 15, no. 1 (1999): 101-107. https://doi.org/10.3892/ijo.15.1.101