Expression and secretion of TGF-beta isoforms and expression of TGF-beta-receptors I, II and III in normal and neoplastic human breast.

  • Authors:
    • D Chakravarthy
    • A R Green
    • V L Green
    • M J Kerin
    • V Speirs
  • View Affiliations

  • Published online on: July 1, 1999     https://doi.org/10.3892/ijo.15.1.187
  • Pages: 187-281
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Abstract

We investigated gene expression of the TGF-beta signalling system (including peptides and receptors) in normal and malignant breast tissue. Additionally, gene and protein expression was determined in a series of primary epithelial and stromal cultures derived from these tissues. TGF-beta isoforms and their receptors were expressed by both tissue sets, however the percentage of samples expressing each transcript varied. In normal breast, both TGF-beta1 and TGF-beta3 were found in most samples (88 and 89% respectively), with fewer expressing TGF-beta2 (68%). A similar pattern was evident in the tumours. Type I receptor of TGF-beta was constitutively expressed in normal breast and observed in most tumours (90%). Type II and III receptors of TGF-beta were expressed less frequently, although the type II receptor was mainly expressed by tumours (P=0. 0075). All primary cultures produced TGF-beta1 and TGF-beta2. Comparing respective cell populations, tumour stromal cells produced significantly more TGF-beta1 than those derived from normal breast (P<0.0001). Linear regression analysis showed stromal cultures derived from breast tumours exhibited a strong positive correlation (r=0.976) in the production of TGF-beta1 and TGF-beta2. Thus, TGF-beta and TGF-beta-receptors are widely and differentially expressed by normal and malignant breast and secretion of this peptide by epithelial and stromal cultures, in particular those derived from tumours, confirms its potential as an autocrine/paracrine regulator in breast cancer.

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Jul 1999
Volume 15 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Chakravarthy D, Green A, Green V, Kerin M and Speirs V: Expression and secretion of TGF-beta isoforms and expression of TGF-beta-receptors I, II and III in normal and neoplastic human breast.. Int J Oncol 15: 187-281, 1999.
APA
Chakravarthy, D., Green, A., Green, V., Kerin, M., & Speirs, V. (1999). Expression and secretion of TGF-beta isoforms and expression of TGF-beta-receptors I, II and III in normal and neoplastic human breast.. International Journal of Oncology, 15, 187-281. https://doi.org/10.3892/ijo.15.1.187
MLA
Chakravarthy, D., Green, A., Green, V., Kerin, M., Speirs, V."Expression and secretion of TGF-beta isoforms and expression of TGF-beta-receptors I, II and III in normal and neoplastic human breast.". International Journal of Oncology 15.1 (1999): 187-281.
Chicago
Chakravarthy, D., Green, A., Green, V., Kerin, M., Speirs, V."Expression and secretion of TGF-beta isoforms and expression of TGF-beta-receptors I, II and III in normal and neoplastic human breast.". International Journal of Oncology 15, no. 1 (1999): 187-281. https://doi.org/10.3892/ijo.15.1.187