Loss of tumorigenicity of human breast cancer cells engineered to produce IL-2, IL-4 or GM-CSF in nude mice.

  • Authors:
    • T Maeda
    • H Matsubara
    • M Sugaya
    • Y Miyazawa
    • Y Gunji
    • T Ochiai
    • S Sakiyama
    • M Tagawa
  • View Affiliations

  • Published online on: November 1, 1999     https://doi.org/10.3892/ijo.15.5.943
  • Pages: 943-950
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Abstract

Human breast cancer cells (OCUB-M), retrovirally transduced with granulocyte macrophage-colony stimulating factor (GM-CSF), interleukin-2 (IL-2) or IL-4 gene were examined for their antitumor activities in nude mice. Although cell proliferation rates in vitro of these cytokine-producing cells were not significantly different from that of wild-type cells, nude mice that were subcutaneously inoculated with cytokine-producing cells did not develop tumors in contrast to mice that were injected with wild-type cells. Injection of GM-CSF-producing cells into the vicinity of growing wild-type tumors retarded subsequent growth of wild-type tumors. Histological examination of tumors which received GM-CSF-producing cells revealed marked infiltration of mononuclear cells around the tumors. Irradiation of cytokine-producing cells diminished their proliferation capacity but production of cytokine(s) was retained. Therefore, inoculation of irradiated cytokine producer cells into growing tumors can be used as a therapeutic maneuver for breast cancer.

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Nov 1999
Volume 15 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Maeda T, Matsubara H, Sugaya M, Miyazawa Y, Gunji Y, Ochiai T, Sakiyama S and Tagawa M: Loss of tumorigenicity of human breast cancer cells engineered to produce IL-2, IL-4 or GM-CSF in nude mice.. Int J Oncol 15: 943-950, 1999
APA
Maeda, T., Matsubara, H., Sugaya, M., Miyazawa, Y., Gunji, Y., Ochiai, T. ... Tagawa, M. (1999). Loss of tumorigenicity of human breast cancer cells engineered to produce IL-2, IL-4 or GM-CSF in nude mice.. International Journal of Oncology, 15, 943-950. https://doi.org/10.3892/ijo.15.5.943
MLA
Maeda, T., Matsubara, H., Sugaya, M., Miyazawa, Y., Gunji, Y., Ochiai, T., Sakiyama, S., Tagawa, M."Loss of tumorigenicity of human breast cancer cells engineered to produce IL-2, IL-4 or GM-CSF in nude mice.". International Journal of Oncology 15.5 (1999): 943-950.
Chicago
Maeda, T., Matsubara, H., Sugaya, M., Miyazawa, Y., Gunji, Y., Ochiai, T., Sakiyama, S., Tagawa, M."Loss of tumorigenicity of human breast cancer cells engineered to produce IL-2, IL-4 or GM-CSF in nude mice.". International Journal of Oncology 15, no. 5 (1999): 943-950. https://doi.org/10.3892/ijo.15.5.943