Modulation of growth of human prostate cancer cells by the N-acetylcysteine conjugate of phenethyl isothiocyanate.

  • Authors:
    • J W Chiao
    • F Chung
    • J Krzeminski
    • S Amin
    • R Arshad
    • T Ahmed
    • C C Conaway
  • View Affiliations

  • Published online on: June 1, 2000     https://doi.org/10.3892/ijo.16.6.1215
  • Pages: 1215-1224
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Abstract

There is growing evidence that thiol conjugates of isothiocyanates present in cruciferous vegetables are effective cancer chemopreventive and potentially active therapeutic agents. The effects of the N-acetylcysteine conjugate of phenethyl isothiocyanate (PEITC-NAC) on tumor cell growth were analyzed in human prostate cancer cell lines LNCaP, androgen-dependent, and DU-145, androgen-independent. Exposure of the cells to PEITC-NAC at high concentrations caused cytolysis, while at lower concentrations PEITC-NAC mediated a dose-dependent growth modulation, with reduction of DNA synthesis and growth rate, inhibition of clonogenicity and induction of apoptosis in both types of prostate cancer cells. PEITC-NAC decreased cells in S and G2M phases of cell cycle, blocking cells entering replicating phases. In parallel, a significant enhancement of cells expressing the cell cycle regulator p21 as well as its intensity was determined using a fluorescent antibody technique. The action of PEITC-NAC was time-dependent, with the magnitude of inhibition increasing to 50-65% after PEITC-NAC exposure for several days. Interaction of tumor cells with dissociation products of PEITC-NAC, PEITC and NAC, are proposed as the mechanism of growth regulation.

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Jun 2000
Volume 16 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Chiao J, Chung F, Krzeminski J, Amin S, Arshad R, Ahmed T and Conaway C: Modulation of growth of human prostate cancer cells by the N-acetylcysteine conjugate of phenethyl isothiocyanate.. Int J Oncol 16: 1215-1224, 2000
APA
Chiao, J., Chung, F., Krzeminski, J., Amin, S., Arshad, R., Ahmed, T., & Conaway, C. (2000). Modulation of growth of human prostate cancer cells by the N-acetylcysteine conjugate of phenethyl isothiocyanate.. International Journal of Oncology, 16, 1215-1224. https://doi.org/10.3892/ijo.16.6.1215
MLA
Chiao, J., Chung, F., Krzeminski, J., Amin, S., Arshad, R., Ahmed, T., Conaway, C."Modulation of growth of human prostate cancer cells by the N-acetylcysteine conjugate of phenethyl isothiocyanate.". International Journal of Oncology 16.6 (2000): 1215-1224.
Chicago
Chiao, J., Chung, F., Krzeminski, J., Amin, S., Arshad, R., Ahmed, T., Conaway, C."Modulation of growth of human prostate cancer cells by the N-acetylcysteine conjugate of phenethyl isothiocyanate.". International Journal of Oncology 16, no. 6 (2000): 1215-1224. https://doi.org/10.3892/ijo.16.6.1215