TNF-alpha induces apoptosis mediated by AEBSF-sensitive serine protease(s) that may involve upstream caspase-3/CPP32 protease activation in a human gastric cancer cell line.

  • Authors:
    • I C Park
    • M J Park
    • T B Choe
    • J J Jang
    • S I Hong
    • S H Lee
  • View Affiliations

  • Published online on: June 1, 2000     https://doi.org/10.3892/ijo.16.6.1243
  • Pages: 1243-1251
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Abstract

In the present study, we investigated the role of caspase-3/CPP32 and serine protease(s) in cell death induced by TNF-alpha in SNU-16 human gastric adenocarcinoma cells. Apoptosis induced in SNU-16 cells by TNF-alpha was accompanied by the activation of caspase-3/CPP32. After treatment with TNF-alpha, PKCdelta cleaved to its characteristic 40 kDa fragment in a caspase-3/CPP32 dependent manner. Incubation with z-DEVD-fmk completely abrogated TNF-alpha-induced DNA fragmentation, indicating that activation of caspase-3/CPP32 was crucially involved in TNF-alpha-induced apoptosis. In addition, serine protease inhibitor, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), clearly inhibited all the features of apoptosis including DNA fragmentation and chromatin condensation. Furthermore, in the AEBSF treated SNU-16 cells, only intact PKCdelta was detected by immunoblot analysis, suggesting that activation of caspase-3/CPP32 was blocked. Thus, the AEBSF-sensitive step may involve an upstream caspase-3/CPP32 protease activation. Taken together, these results suggest that both caspase-3/CPP32 and serine protease(s) are activated and play an important role in TNF-alpha induced apoptosis in SNU-16 cells.

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Jun 2000
Volume 16 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park I, Park M, Choe T, Jang J, Hong S and Lee S: TNF-alpha induces apoptosis mediated by AEBSF-sensitive serine protease(s) that may involve upstream caspase-3/CPP32 protease activation in a human gastric cancer cell line.. Int J Oncol 16: 1243-1251, 2000
APA
Park, I., Park, M., Choe, T., Jang, J., Hong, S., & Lee, S. (2000). TNF-alpha induces apoptosis mediated by AEBSF-sensitive serine protease(s) that may involve upstream caspase-3/CPP32 protease activation in a human gastric cancer cell line.. International Journal of Oncology, 16, 1243-1251. https://doi.org/10.3892/ijo.16.6.1243
MLA
Park, I., Park, M., Choe, T., Jang, J., Hong, S., Lee, S."TNF-alpha induces apoptosis mediated by AEBSF-sensitive serine protease(s) that may involve upstream caspase-3/CPP32 protease activation in a human gastric cancer cell line.". International Journal of Oncology 16.6 (2000): 1243-1251.
Chicago
Park, I., Park, M., Choe, T., Jang, J., Hong, S., Lee, S."TNF-alpha induces apoptosis mediated by AEBSF-sensitive serine protease(s) that may involve upstream caspase-3/CPP32 protease activation in a human gastric cancer cell line.". International Journal of Oncology 16, no. 6 (2000): 1243-1251. https://doi.org/10.3892/ijo.16.6.1243