Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.

  • Authors:
    • K Okazaki
    • Y Nakayama
    • K Shibao
    • K Hirata
    • T Sako
    • N Nagata
    • Y Kuroda
    • H Itoh
  • View Affiliations

  • Published online on: July 1, 2000     https://doi.org/10.3892/ijo.17.1.39
  • Pages: 39-84
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Abstract

A new human colon cancer cell line (PMF-ko14) derived from a peritoneal disseminated tumor has been established and maintained for over 25 months. In tissue culture, the cells grew in a mainly monolayered sheet with a population doubling time of about 27 h. Chromosome counts at the 60th passage ranged from 79 to 84 with a modal number of 83. Flow cytometry of the cell surface antigen expression indicated that CD49b, CD29, carcinoembryonic antigen (CEA), sialyl Lewis a (sLea), and CD49c were positive in more than 70% of the cells. The nude mouse xenograft models indicated are: subcutaneous or intraperitoneal injection model, spleen injection-liver metastasis model, and orthotopic implantation-spontaneous metastasis model. As PMF-ko14 has highly metastatic activity it should prove to be a useful tool for research in biological behavior of metastatic colon cancer.

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Jul 2000
Volume 17 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Okazaki K, Nakayama Y, Shibao K, Hirata K, Sako T, Nagata N, Kuroda Y and Itoh H: Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.. Int J Oncol 17: 39-84, 2000
APA
Okazaki, K., Nakayama, Y., Shibao, K., Hirata, K., Sako, T., Nagata, N. ... Itoh, H. (2000). Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.. International Journal of Oncology, 17, 39-84. https://doi.org/10.3892/ijo.17.1.39
MLA
Okazaki, K., Nakayama, Y., Shibao, K., Hirata, K., Sako, T., Nagata, N., Kuroda, Y., Itoh, H."Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.". International Journal of Oncology 17.1 (2000): 39-84.
Chicago
Okazaki, K., Nakayama, Y., Shibao, K., Hirata, K., Sako, T., Nagata, N., Kuroda, Y., Itoh, H."Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.". International Journal of Oncology 17, no. 1 (2000): 39-84. https://doi.org/10.3892/ijo.17.1.39