Expression analysis and mutational screening of the epithelium-specific ets gene-1 (ESE-1) in patients with squamous anal cancer.

  • Authors:
    • C Zucchini
    • P Strippoli
    • G Rosati
    • M Del Governatore
    • E Milano
    • G Ugolini
    • R Solmi
    • G Mattei
    • A Caira
    • S Zanotti
    • P Carinci
    • L Valvassori
  • View Affiliations

  • Published online on: August 1, 2000     https://doi.org/10.3892/ijo.17.2.265
  • Pages: 265-335
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Abstract

To investigate whether ESE-1 gene abnormalities are involved in alterations of epithelial cell differentiation in squamous anal cancer ESE-1 expression and structure were screened in six patients by reverse transcriptase-polymerase chain reaction (RT-PCR) and automated sequence analysis. The complete cDNA of isoform ESE-1b was always expressed and correctly spliced, with single nucleotide polymorphism being observed in two cases. Presence of ESE-1b point mutations was excluded. Expression of SPRR2A and ENDOA/CK8, two epithelium-specific ESE-1 target genes, were revealed by RT-PCR in all cases. This first report of expression of ESE-1, and of SPRR2A and ENDOA/CK8 (both related to terminal differentiation in different types of epithelia lining) in anal cancer excludes the hypothesis that these genes influenced carcinogenesis in our patients. Despite selecting of patients without clinical evidence of HPV infection, PCR consistently revealed HPV-16 DNA, highlighting the importance of HPV infection in anal cancer.

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Aug 2000
Volume 17 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zucchini C, Strippoli P, Rosati G, Del Governatore M, Milano E, Ugolini G, Solmi R, Mattei G, Caira A, Zanotti S, Zanotti S, et al: Expression analysis and mutational screening of the epithelium-specific ets gene-1 (ESE-1) in patients with squamous anal cancer.. Int J Oncol 17: 265-335, 2000.
APA
Zucchini, C., Strippoli, P., Rosati, G., Del Governatore, M., Milano, E., Ugolini, G. ... Valvassori, L. (2000). Expression analysis and mutational screening of the epithelium-specific ets gene-1 (ESE-1) in patients with squamous anal cancer.. International Journal of Oncology, 17, 265-335. https://doi.org/10.3892/ijo.17.2.265
MLA
Zucchini, C., Strippoli, P., Rosati, G., Del Governatore, M., Milano, E., Ugolini, G., Solmi, R., Mattei, G., Caira, A., Zanotti, S., Carinci, P., Valvassori, L."Expression analysis and mutational screening of the epithelium-specific ets gene-1 (ESE-1) in patients with squamous anal cancer.". International Journal of Oncology 17.2 (2000): 265-335.
Chicago
Zucchini, C., Strippoli, P., Rosati, G., Del Governatore, M., Milano, E., Ugolini, G., Solmi, R., Mattei, G., Caira, A., Zanotti, S., Carinci, P., Valvassori, L."Expression analysis and mutational screening of the epithelium-specific ets gene-1 (ESE-1) in patients with squamous anal cancer.". International Journal of Oncology 17, no. 2 (2000): 265-335. https://doi.org/10.3892/ijo.17.2.265