Overexpression of P-glycoprotein is associated with a decreased mitochondrial transmembrane potential in doxorubicin-selected K562 human leukemia cells.

  • Authors:
    • A A Shtil
    • T M Grinchuk
    • L Tee
    • E B Mechetner
    • T N Ignatova
  • View Affiliations

  • Published online on: August 1, 2000     https://doi.org/10.3892/ijo.17.2.387
  • Pages: 387-479
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Abstract

We tested the hypothesis of whether overexpression of P-glycoprotein (Pgp) could be coupled with changes in specific mechanisms of antioxidant defense (in particular, transition of mitochondrial transmembrane potential, MTP) in tumor cells chronically exposed to anticancer drugs known to exert their cytotoxicity via oxidative stress. We show elevation of Pgp associated with decreased MTP in doxorubicin-selected K562Dox subline as compared with parental K562 cells. The low MTP was not due to a fewer number of mitochondria in K562Dox cells, nor was it associated with altered content of Bcl-XL protein. We discuss a model for coordinated up-regulation of Pgp and MTP transition in cells that survived chemotherapy-induced oxidative stress.

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Aug 2000
Volume 17 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Shtil A, Grinchuk T, Tee L, Mechetner E and Ignatova T: Overexpression of P-glycoprotein is associated with a decreased mitochondrial transmembrane potential in doxorubicin-selected K562 human leukemia cells.. Int J Oncol 17: 387-479, 2000
APA
Shtil, A., Grinchuk, T., Tee, L., Mechetner, E., & Ignatova, T. (2000). Overexpression of P-glycoprotein is associated with a decreased mitochondrial transmembrane potential in doxorubicin-selected K562 human leukemia cells.. International Journal of Oncology, 17, 387-479. https://doi.org/10.3892/ijo.17.2.387
MLA
Shtil, A., Grinchuk, T., Tee, L., Mechetner, E., Ignatova, T."Overexpression of P-glycoprotein is associated with a decreased mitochondrial transmembrane potential in doxorubicin-selected K562 human leukemia cells.". International Journal of Oncology 17.2 (2000): 387-479.
Chicago
Shtil, A., Grinchuk, T., Tee, L., Mechetner, E., Ignatova, T."Overexpression of P-glycoprotein is associated with a decreased mitochondrial transmembrane potential in doxorubicin-selected K562 human leukemia cells.". International Journal of Oncology 17, no. 2 (2000): 387-479. https://doi.org/10.3892/ijo.17.2.387