PTEN/MMAC1 expression in esophageal squamous cell carcinomas.

  • Authors:
    • Y Ding
    • Y Shimada
    • M Kano
    • A Itami
    • A Kawabe
    • M Maeda
    • Z Li
    • T Hong
    • F Sato
    • J Kaganoi
    • M Imamura
  • View Affiliations

  • Published online on: October 1, 2000     https://doi.org/10.3892/ijo.17.4.695
  • Pages: 695-704
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Abstract

The PTEN/MMAC1 gene at 10q23.3 is a novel tumor suppressor gene candidate. Various kinds of tumors have mutations in this gene. However, in some cancers PTEN/MMAC1 gene may be inactivated by mechanisms other than gene deletion and mutation, including promoter methylation or translational modification. The aim of this study was to determine whether there are abnormalities in the expression of PTEN/MMAC1 gene in esophageal cancer. Reverse transcription polymerase chain reaction and western blot were used to examine PTEN/MMAC1 expression in human esophageal squamous cell lines and normal cultured esophageal epithelial cells. Immunohistochemical staining was carried out to detect PTEN/MMAC1 expression in surgically resected esophageal squamous cell carcinomas and normal esophageal epithelium. All 30 esophageal cancer cell lines (KYSE series) and normal cultured esophageal epithelial cells expressed PTEN/MMAC1 mRNA. Western blot analysis confirmed that all the cell lines expressed PTEN/MMAC1 protein and there was no difference in expression level. Immunohistochemical study showed that the PTEN/MMAC1 protein was localized dominantly in the cytoplasm and strongly stained in all 42 surgically resected esophageal squamous cell carcinomas and normal esophageal epithelium. PTEN/MMAC1 is rarely associated with esophageal squamous cell carcinoma carcinogenesis.

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Oct 2000
Volume 17 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ding Y, Shimada Y, Kano M, Itami A, Kawabe A, Maeda M, Li Z, Hong T, Sato F, Kaganoi J, Kaganoi J, et al: PTEN/MMAC1 expression in esophageal squamous cell carcinomas.. Int J Oncol 17: 695-704, 2000
APA
Ding, Y., Shimada, Y., Kano, M., Itami, A., Kawabe, A., Maeda, M. ... Imamura, M. (2000). PTEN/MMAC1 expression in esophageal squamous cell carcinomas.. International Journal of Oncology, 17, 695-704. https://doi.org/10.3892/ijo.17.4.695
MLA
Ding, Y., Shimada, Y., Kano, M., Itami, A., Kawabe, A., Maeda, M., Li, Z., Hong, T., Sato, F., Kaganoi, J., Imamura, M."PTEN/MMAC1 expression in esophageal squamous cell carcinomas.". International Journal of Oncology 17.4 (2000): 695-704.
Chicago
Ding, Y., Shimada, Y., Kano, M., Itami, A., Kawabe, A., Maeda, M., Li, Z., Hong, T., Sato, F., Kaganoi, J., Imamura, M."PTEN/MMAC1 expression in esophageal squamous cell carcinomas.". International Journal of Oncology 17, no. 4 (2000): 695-704. https://doi.org/10.3892/ijo.17.4.695