A method for detecting antibody internalization in drug targeting.

  • Authors:
    • J Jaime
    • M Pagé
  • View Affiliations

  • Published online on: October 1, 2000     https://doi.org/10.3892/ijo.17.4.835
  • Pages: 835-844
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Abstract

BCM43/2E5 monoclonal antibody with a specificity for human ovarian carcinoma was evaluated for its binding and internalization behaviour on both fixed and live cells. Internalization of this antibody by the targeted cells was demonstrated by ELISA, flow cytometry and immunoperoxidase. At reduced temperatures or in the presence of electron transfer blockers, internalization of bound antibody was prevented. This method is rapid and reproducible and may be used efficiently for the selection of antibodies suitable for drug targeting in cancer therapy.

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Oct 2000
Volume 17 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Jaime J and Jaime J: A method for detecting antibody internalization in drug targeting.. Int J Oncol 17: 835-844, 2000
APA
Jaime, J., & Jaime, J. (2000). A method for detecting antibody internalization in drug targeting.. International Journal of Oncology, 17, 835-844. https://doi.org/10.3892/ijo.17.4.835
MLA
Jaime, J., Pagé, M."A method for detecting antibody internalization in drug targeting.". International Journal of Oncology 17.4 (2000): 835-844.
Chicago
Jaime, J., Pagé, M."A method for detecting antibody internalization in drug targeting.". International Journal of Oncology 17, no. 4 (2000): 835-844. https://doi.org/10.3892/ijo.17.4.835