Protein kinase C activation by PMA rapidly induces apoptosis through caspase-3/CPP32 and serine protease(s) in a gastric cancer cell line

  • Authors:
    • In-Chul Park
    • Myung-Jin Park
    • Chang-Hun Rhee
    • Jong-Inn Lee
    • Tae-Boo Choe
    • Ja-Jun Jang
    • Seung-Hoon Lee
    • Seok-Il Hong
  • View Affiliations

  • Published online on: May 1, 2001     https://doi.org/10.3892/ijo.18.5.1077
  • Pages: 1077-1083
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Abstract

Phorbol 12-myristate 13-acetate (PMA) rapidly induced cell death in SNU-16 gastric adenocarcinoma cells. DNA ladder formation and caspase-3/CPP32 activation were observed in PMA treated cells indicating that PMA induces apoptosis. z-DEVD-fmk, specific inhibitor of caspase-3/CPP32, inhibited the induction of apoptosis by PMA, demonstrating that caspase/CPP32 are critically involved in PMA-induced apoptosis. The serine protein inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride effectively blocked apoptosis, and also prevented caspase-3/CPP32 activation. Go6983, a specific inhibitor of PKC, almost completely suppressed apoptosis and caspase-3/CPP32 activation. Furthermore, 1,2-dihexanoyl-sn-glycerol, an endogenous activator of PKC, induced apoptosis detected by DNA fragmentation and Hoechst 33258 nuclear staining. From these results, we conclude that PMA is not only a tumor promoter, but can also induce apoptosis in gastric cancer cells. PMA-induced apoptosis appears to be mediated through activation of protein kinase C, and the activation of serine protease(s) and caspase-3/CPP32 may be the molecular mechanisms by which PMA induces apoptosis.

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May 2001
Volume 18 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park I, Park M, Rhee C, Lee J, Choe T, Jang J, Lee S and Hong S: Protein kinase C activation by PMA rapidly induces apoptosis through caspase-3/CPP32 and serine protease(s) in a gastric cancer cell line. Int J Oncol 18: 1077-1083, 2001
APA
Park, I., Park, M., Rhee, C., Lee, J., Choe, T., Jang, J. ... Hong, S. (2001). Protein kinase C activation by PMA rapidly induces apoptosis through caspase-3/CPP32 and serine protease(s) in a gastric cancer cell line. International Journal of Oncology, 18, 1077-1083. https://doi.org/10.3892/ijo.18.5.1077
MLA
Park, I., Park, M., Rhee, C., Lee, J., Choe, T., Jang, J., Lee, S., Hong, S."Protein kinase C activation by PMA rapidly induces apoptosis through caspase-3/CPP32 and serine protease(s) in a gastric cancer cell line". International Journal of Oncology 18.5 (2001): 1077-1083.
Chicago
Park, I., Park, M., Rhee, C., Lee, J., Choe, T., Jang, J., Lee, S., Hong, S."Protein kinase C activation by PMA rapidly induces apoptosis through caspase-3/CPP32 and serine protease(s) in a gastric cancer cell line". International Journal of Oncology 18, no. 5 (2001): 1077-1083. https://doi.org/10.3892/ijo.18.5.1077