Effect of cigarette smoke exposure on the modulation of 8-oxo-2'-deoxyguanosine in rat lungs as analyzed by 32P-postlabeling and HPLC-ECD

  • Authors:
    • Jamal M. Arif
    • Manicka V. Vadhanam
    • Anton J.L. De Groot
    • Albert A. Van Zeeland
    • C. Gary Gairola
    • Ramesh C. Gupta
  • View Affiliations

  • Published online on: October 1, 2001     https://doi.org/10.3892/ijo.19.4.763
  • Pages: 763-766
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Abstract

Cigarette smoke contains several oxidants and free radicals. In the present study, we examined the formation of 8-oxo-2'-deoxyguanosine (8-oxodG) in the lungs of female Sprague-Dawley rats exposed to side-stream cigarette smoke for 6 h a day, 7 days a week for 1, 2, 4 and 12 weeks in a whole body-exposure system. The samples were analyzed for 8-oxodG by 32P-postlabeling-TLC enrichment and HPLC-ECD techniques to confirm and compare results. Animals were sacrificed 15 h after the cessation of smoke exposure and lung DNA was isolated by phenol/Sevag extractions in the presence of the free radical traps, 8-hydroxyquinoline (6.8 mM) and N-t-butyl-α-phenyl nitrone (500 μM) to minimize artifactual formation of 8-oxodG during sample work up. Analysis of lung DNA by 32P-postlabeling-TLC showed 8-oxodG levels (mean ± SE) of 1.45±0.24, 2.68±0.65, 2.23±0.28 and 2.93±0.54 per 106 nucleotides after 1, 2, 4 and 12 weeks of smoke exposure. The respective values in sham-treated rats were 2.76±0.19, 3.69±0.20, 1.44±0.43 and 2.84±0.45 per 106 nucleotides, suggesting no significant effect of smoke exposure on tissue levels of 8-oxodG. HPLC-ECD procedure yielded slightly higher values for 8-oxodG in all groups, however, again significant differences between sham and smoke-exposed groups were not detected. It is concluded that the chronic exposure to side-stream cigarette smoke does not enhance the formation of 8-oxodG in rat lungs.

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October 2001
Volume 19 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Arif JM, Vadhanam MV, De Groot AJ, Van Zeeland AA, Gairola CG and Gupta RC: Effect of cigarette smoke exposure on the modulation of 8-oxo-2'-deoxyguanosine in rat lungs as analyzed by 32P-postlabeling and HPLC-ECD. Int J Oncol 19: 763-766, 2001
APA
Arif, J.M., Vadhanam, M.V., De Groot, A.J., Van Zeeland, A.A., Gairola, C.G., & Gupta, R.C. (2001). Effect of cigarette smoke exposure on the modulation of 8-oxo-2'-deoxyguanosine in rat lungs as analyzed by 32P-postlabeling and HPLC-ECD. International Journal of Oncology, 19, 763-766. https://doi.org/10.3892/ijo.19.4.763
MLA
Arif, J. M., Vadhanam, M. V., De Groot, A. J., Van Zeeland, A. A., Gairola, C. G., Gupta, R. C."Effect of cigarette smoke exposure on the modulation of 8-oxo-2'-deoxyguanosine in rat lungs as analyzed by 32P-postlabeling and HPLC-ECD". International Journal of Oncology 19.4 (2001): 763-766.
Chicago
Arif, J. M., Vadhanam, M. V., De Groot, A. J., Van Zeeland, A. A., Gairola, C. G., Gupta, R. C."Effect of cigarette smoke exposure on the modulation of 8-oxo-2'-deoxyguanosine in rat lungs as analyzed by 32P-postlabeling and HPLC-ECD". International Journal of Oncology 19, no. 4 (2001): 763-766. https://doi.org/10.3892/ijo.19.4.763