Multiple lysosomal trafficking phenotypes in metastatic mouse mammary tumor cell lines

  • Authors:
    • Kai Tao
    • Jin Li
    • Jason Warner
    • Kathy MacLeod
    • Fred R. Miller
    • G. Gary Sahagian
  • View Affiliations

  • Published online on: December 1, 2001     https://doi.org/10.3892/ijo.19.6.1333
  • Pages: 1333-1339
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Abstract

Although altered synthesis and trafficking of lysosomal proteins and their receptors are associated with a wide range of human and rodent malignancies, the basis for their involvement remains obscure. Here we describe findings on a set of mouse mammary tumor cell lines that we are using as a model to study the role of these proteins in oncogenesis and tumor progression. Three distinct proteinase-secreting phenotypes were identified among the metastatic cell lines of the set. Two phenotypes displayed a high level of secretion of cathepsin L and the third was characterized by elevated secretion of matrix metalloproteinase 9 (MMP-9). The two cathepsin L-secreting phenotypes were distinct in that they displayed differences in cathepsin trafficking, expression of mannose 6-phosphate/insulin-like growth factor receptor and expression of proliferin, a mannose-phosphorylated angiogenic factor. Although cells representing all three phenotypes are capable of dissemination to distant organs when implanted into mouse mammary glands, only cells with the MMP-9 phenotype were found to be capable of direct intravasation. These findings indicate that multiple proteinase-secreting phenotypes can arise from the same tumor and suggest that cathepsin L and other lysosomal proteins may play a role in dissemination of tumor cells via the lymphatic system.

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December 2001
Volume 19 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tao K, Li J, Warner J, MacLeod K, Miller FR and Sahagian GG: Multiple lysosomal trafficking phenotypes in metastatic mouse mammary tumor cell lines. Int J Oncol 19: 1333-1339, 2001
APA
Tao, K., Li, J., Warner, J., MacLeod, K., Miller, F.R., & Sahagian, G.G. (2001). Multiple lysosomal trafficking phenotypes in metastatic mouse mammary tumor cell lines. International Journal of Oncology, 19, 1333-1339. https://doi.org/10.3892/ijo.19.6.1333
MLA
Tao, K., Li, J., Warner, J., MacLeod, K., Miller, F. R., Sahagian, G. G."Multiple lysosomal trafficking phenotypes in metastatic mouse mammary tumor cell lines". International Journal of Oncology 19.6 (2001): 1333-1339.
Chicago
Tao, K., Li, J., Warner, J., MacLeod, K., Miller, F. R., Sahagian, G. G."Multiple lysosomal trafficking phenotypes in metastatic mouse mammary tumor cell lines". International Journal of Oncology 19, no. 6 (2001): 1333-1339. https://doi.org/10.3892/ijo.19.6.1333