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PREPARATION OF SUSPENSIONS OF HUMAN CERVICAL EPITHELIAL-CELLS SUITABLE FOR BIOCHEMICAL, CYTOCHEMICAL AND IMMUNOCYTOCHEMICAL INVESTIGATIONS

  • Authors:
    • TF SLATER
    • JS BEESLEY
    • A FLATMAN
    • RH HAMMOND
    • SK JONAS

  • View Affiliations

  • Published online on: February 1, 1993     https://doi.org/10.3892/ijo.2.2.233
  • Pages: 233-236
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

A simple gradient centrifuging method that separates epithelial cells from suspensions of material obtained by conventional cervical sampling procedures, is described. Heterogeneous cell suspensions were disaggregated by syringing and placed on a discontinuous density gradient. Following centrifugation up to 5 bands of, cells could be collected. The three central bands of epithelial cells corresponded to a density range between 1.035g/ml and 1.055g/ml. The cell samples so obtained are suitable for biochemical, cytochemical and immunocytochemical investigations.

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February 1993
Volume 2 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 5.2
  • Ranked Oncology
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  • CiteScore: 11.4
  • CiteScore Rank: Q1: #40/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 1.125
  • SCImago Journal Rank (SJR): 1.105
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Spandidos Publications style
SLATER T, BEESLEY J, FLATMAN A, HAMMOND R and JONAS S: PREPARATION OF SUSPENSIONS OF HUMAN CERVICAL EPITHELIAL-CELLS SUITABLE FOR BIOCHEMICAL, CYTOCHEMICAL AND IMMUNOCYTOCHEMICAL INVESTIGATIONS. Int J Oncol 2: 233-236, 1993
APA
SLATER, T., BEESLEY, J., FLATMAN, A., HAMMOND, R., & JONAS, S. (1993). PREPARATION OF SUSPENSIONS OF HUMAN CERVICAL EPITHELIAL-CELLS SUITABLE FOR BIOCHEMICAL, CYTOCHEMICAL AND IMMUNOCYTOCHEMICAL INVESTIGATIONS. International Journal of Oncology, 2, 233-236. https://doi.org/10.3892/ijo.2.2.233
MLA
SLATER, T., BEESLEY, J., FLATMAN, A., HAMMOND, R., JONAS, S."PREPARATION OF SUSPENSIONS OF HUMAN CERVICAL EPITHELIAL-CELLS SUITABLE FOR BIOCHEMICAL, CYTOCHEMICAL AND IMMUNOCYTOCHEMICAL INVESTIGATIONS". International Journal of Oncology 2.2 (1993): 233-236.
Chicago
SLATER, T., BEESLEY, J., FLATMAN, A., HAMMOND, R., JONAS, S."PREPARATION OF SUSPENSIONS OF HUMAN CERVICAL EPITHELIAL-CELLS SUITABLE FOR BIOCHEMICAL, CYTOCHEMICAL AND IMMUNOCYTOCHEMICAL INVESTIGATIONS". International Journal of Oncology 2, no. 2 (1993): 233-236. https://doi.org/10.3892/ijo.2.2.233