Dominant-negative activity of a Brca1 truncation mutant: Effects on proliferation, tumorigenicity in vivo, and chemosensitivity in a mouse ovarian cancer cell line

  • Authors:
    • V. Sylvain
    • S. Lafarge
    • Y.-J. Bignon
  • View Affiliations

  • Published online on: April 1, 2002     https://doi.org/10.3892/ijo.20.4.845
  • Pages: 845-853
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Abstract

In order to generate an in vitro mouse model for the study of human ovarian cancers, we compared the effects of a truncated Brca1 mutant expression on cellular phenotype with those of a full-length sense and antisense Brca1 expression in the ID-8 mouse epithelial ovarian cancer cell line. The examined cellular processes include proliferation, tumorigenicity in syngeneic mice in vivo and sensitivity/resistance to several cytotoxic drugs. We found that the expression of a spontaneous truncated Brca1 mutant in ID-8 cells which contain two endogenous wild-type Brca1 alleles led to a dominant-negative effect of Brca1, demonstrated by an increase in tumorigenicity in vivo and in chemosensitivity. Expression of a truncated Brca1 mutant in a mouse epithelial ovarian cancer cell line could thus provide a powerful in vitro model for the study of human BRCA1-related ovarian tumorigenesis.

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April 2002
Volume 20 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sylvain V, Lafarge S and Bignon Y: Dominant-negative activity of a Brca1 truncation mutant: Effects on proliferation, tumorigenicity in vivo, and chemosensitivity in a mouse ovarian cancer cell line. Int J Oncol 20: 845-853, 2002
APA
Sylvain, V., Lafarge, S., & Bignon, Y. (2002). Dominant-negative activity of a Brca1 truncation mutant: Effects on proliferation, tumorigenicity in vivo, and chemosensitivity in a mouse ovarian cancer cell line. International Journal of Oncology, 20, 845-853. https://doi.org/10.3892/ijo.20.4.845
MLA
Sylvain, V., Lafarge, S., Bignon, Y."Dominant-negative activity of a Brca1 truncation mutant: Effects on proliferation, tumorigenicity in vivo, and chemosensitivity in a mouse ovarian cancer cell line". International Journal of Oncology 20.4 (2002): 845-853.
Chicago
Sylvain, V., Lafarge, S., Bignon, Y."Dominant-negative activity of a Brca1 truncation mutant: Effects on proliferation, tumorigenicity in vivo, and chemosensitivity in a mouse ovarian cancer cell line". International Journal of Oncology 20, no. 4 (2002): 845-853. https://doi.org/10.3892/ijo.20.4.845