Effect of hypoxia on human seminoma cells

  • Authors:
    • Tomohiro Fujii
    • Takemi Otsuki
    • Takuya Moriya
    • Haruko Sakaguchi
    • Junichi Kurebayashi
    • Kenichiro Yata
    • Masako Uno
    • Tatsuya Kobayashi
    • Takahiro Kimura
    • Yoshimasa Jo
    • Keigo Kinugawa
    • Yoji Furukawa
    • Masaaki Morioka
    • Ayako Ueki
    • Hiroyoshi Tanaka
  • View Affiliations

  • Published online on: May 1, 2002     https://doi.org/10.3892/ijo.20.5.955
  • Pages: 955-962
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Abstract

Since hypoxia has been considered to enhance metastatic potential in solid tumors via a neo-angiogenesis caused by vascular endothelial cell growth factors (VEGFs) induced by hypoxia inducible factor-1α (HIF-1α), the effects of hypoxia on human seminoma cell lines were examined in terms of growth, morphology, gene expression, protein expression and cell cycle perturbation. Growth was inhibited in long-term cultures with morphological changes to the spindle form. The gene expression of VEGF-C was markedly enhanced and the production of VEGF-A increased during hypoxia, although HIF-1α was not upregulated at the protein or message level. Hypoxic culture caused G1 cell cycle arrest with upregulation of the p15/ink4b and p27/Kip1 genes, whereas no increase of apoptotic cells was observed on up-regulation of the heat shock protein (HSP) 70 gene. The adhesion molecules were only slightly altered.

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May 2002
Volume 20 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Fujii T, Otsuki T, Moriya T, Sakaguchi H, Kurebayashi J, Yata K, Uno M, Kobayashi T, Kimura T, Jo Y, Jo Y, et al: Effect of hypoxia on human seminoma cells. Int J Oncol 20: 955-962, 2002
APA
Fujii, T., Otsuki, T., Moriya, T., Sakaguchi, H., Kurebayashi, J., Yata, K. ... Tanaka, H. (2002). Effect of hypoxia on human seminoma cells. International Journal of Oncology, 20, 955-962. https://doi.org/10.3892/ijo.20.5.955
MLA
Fujii, T., Otsuki, T., Moriya, T., Sakaguchi, H., Kurebayashi, J., Yata, K., Uno, M., Kobayashi, T., Kimura, T., Jo, Y., Kinugawa, K., Furukawa, Y., Morioka, M., Ueki, A., Tanaka, H."Effect of hypoxia on human seminoma cells". International Journal of Oncology 20.5 (2002): 955-962.
Chicago
Fujii, T., Otsuki, T., Moriya, T., Sakaguchi, H., Kurebayashi, J., Yata, K., Uno, M., Kobayashi, T., Kimura, T., Jo, Y., Kinugawa, K., Furukawa, Y., Morioka, M., Ueki, A., Tanaka, H."Effect of hypoxia on human seminoma cells". International Journal of Oncology 20, no. 5 (2002): 955-962. https://doi.org/10.3892/ijo.20.5.955