Molecular cloning and characterization of Strabismus 2 (STB2)

  • Authors:
    • Masaru Katoh
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  • Published online on: May 1, 2002     https://doi.org/10.3892/ijo.20.5.993
  • Pages: 993-998
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Abstract

WNT signaling pathway is implicated in carcinogenesis and embryogenesis. WNT signal is transduced to the β-catenin - TCF pathway, the JNK pathway, or the Ca2+-releasing pathway through seven-transmembrane-type WNT receptors encoded by Frizzled (FZD) genes. Xenopus Strabismus (Stbm) is a tetra-spanning transmembrane protein interacting with Dishevelled, and is a negative regulator of the WNT - β-catenin - TCF signaling pathway. STB1/KIAA1215/VANGL2 is a human orthologue of Xenopus Stbm (90.6% total-amino-acid identity). Here, STB2/VANGL1 gene fragments were identified in human genome draft sequences by using bioinformatics, and STB2 cDNAs were isolated by using cDNA-PCR. STB2 gene consisted of at lest 7 exons, and encoded a 524-amino-acid protein with 4 transmembrane domains and the C-terminal Ser/Thr-X-Val motif. Human STB2 was homologous to human STB1 (73.1% total-amino-acid identity) and Xenopus Stbm (72.7% total-amino-acid identity). STB2 gene was clustered with Calsequestrin 2 (CASQ2) gene in tail-to-tail manner (interval less than 5.0 kb), and CASQ2 gene is mapped to human chromosome 1p11-p13.3 or linked to human chromosome 1p13-p21. STB2 mRNAs of 4.8- and 6.8-kb in size were expressed almost ubiquitously in various normal tissues. STB2 mRNA was significantly up-regulated in gastric cancer cell lines MKN28, MKN74, pancreatic cancer cell lines BxPC-3, PSN-1 and Hs766T. On the other hand, STB2 mRNA was significantly down-regulated in a pancreatic cancer cell line AsPC-1. This is the first report on molecular cloning and characterization of STB2.

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May 2002
Volume 20 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Katoh M: Molecular cloning and characterization of Strabismus 2 (STB2). Int J Oncol 20: 993-998, 2002
APA
Katoh, M. (2002). Molecular cloning and characterization of Strabismus 2 (STB2). International Journal of Oncology, 20, 993-998. https://doi.org/10.3892/ijo.20.5.993
MLA
Katoh, M."Molecular cloning and characterization of Strabismus 2 (STB2)". International Journal of Oncology 20.5 (2002): 993-998.
Chicago
Katoh, M."Molecular cloning and characterization of Strabismus 2 (STB2)". International Journal of Oncology 20, no. 5 (2002): 993-998. https://doi.org/10.3892/ijo.20.5.993