MALAT-1: A long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis

  • Authors:
    • Chuan Xu
    • Minhui Yang
    • Jie Tian
    • Xiaoyan Wang
    • Zuguo Li
  • View Affiliations

  • Published online on: April 15, 2011     https://doi.org/10.3892/ijo.2011.1007
  • Pages: 169-175
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Abstract

The human metastasis associated lung adenocarcinoma transcript 1 (MALAT-1) as a long non-coding RNA known to be misregulated in many people who are detected with cancer. Our earlier studies found that MALAT-1 plays a pivotal role in colorectal cancer (CRC) metastasis. In this study, we analyzed the MALAT-1 gene in five fragments. We employed the sequencing process to identify MALAT-1 mutations in the following types of samples: CRC cells (SW620, SW480), normal colorectal tissues, and primary CRC tissues. We were successful in detecting the following mutations: fragment 5434 nt-6951 nt of the MALAT-1 was mutated in SW620 cells, while fragments 5434 nt-6951 nt and 6918 nt-8441 nt of MALAT-1 were mutated in SW480 cancer cells and primary CRC tissues. We over-expressed five fragments of MALAT-1 in the CRC cell line SW480; simultaneously ensuring that MALAT-1 had low expression. Our data illustrated that one of the 5 fragments (6918 nt-8441 nt) located at the 3' end of MALAT-1 plays a pivotal role in the biological processes of cell proliferation, migration and invasion. Based on these observations, we can infer that the 3' end of MALAT-1 is an important biological motif in the invasion and metastasis of CRC cells. We have successfully presented the first evidence that mutations were found on the long non-coding RNA MALAT-1 in CRC. Moreover, long non-coding RNA MALAT-1 has an important biological motif located at the 3' end of MALAT-1 (6918 nt-8441 nt) in CRC. Our study gives a new direction to research primarily focused on exploring the molecular mechanisms occurring during the invasion and metastasis of CRC.

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July 2011
Volume 39 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Xu, C., Yang, M., Tian, J., Wang, X., & Li, Z. (2011). MALAT-1: A long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis. International Journal of Oncology, 39, 169-175. https://doi.org/10.3892/ijo.2011.1007
MLA
Xu, C., Yang, M., Tian, J., Wang, X., Li, Z."MALAT-1: A long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis". International Journal of Oncology 39.1 (2011): 169-175.
Chicago
Xu, C., Yang, M., Tian, J., Wang, X., Li, Z."MALAT-1: A long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis". International Journal of Oncology 39, no. 1 (2011): 169-175. https://doi.org/10.3892/ijo.2011.1007