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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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March 2012 Volume 40 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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An International Open Access Journal Devoted to General Medicine.

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Article

Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA

  • Authors:
    • Christine Challen
    • John J. Anderson
    • Zofia   M.A. Chrzanowska‑Lightowlers
    • Robert N. Lightowlers
    • John Lunec

  • View Affiliations / Copyright

    Affiliations: Northern Institute for Cancer Research, Newcastle University Medical School, Newcastle-upon-Tyne, NE2 4HH, UK, Northern Institute for Cancer Research, Newcastle University Medical School, Newcastle-upon Tyne, NE2 4HH, UK
  • Pages: 851-859
    |
    Published online on: November 21, 2011
       https://doi.org/10.3892/ijo.2011.1267
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Abstract

MDM2 is a 90 kDa nucleo-phosphoprotein that binds p53 and other proteins contributing to its oncogenic properties. Its structure includes an amino proximal p53 binding site, a central acidic domain and a carboxy region which incorporates Zinc and Ring Finger domains suggestive of nucleic acid binding or transcription factor function. It has previously been reported that a bacculovirus expressed MDM2 protein binds RNA in a sequence-specific manner through the Ring Finger domain, however, its ability to bind DNA has yet to be examined. We report here that a bacterially expressed human MDM2 protein binds both DNA as well as the previously defined RNA consensus sequence. DNA binding appears selective and involves the carboxy-terminal domain of the molecule. RNA binding is inhibited by an MDM2 specific antibody, which recognises an epitope within the carboxy region of the protein. Selection cloning and sequence analysis of MDM2 DNA binding sequences, unlike RNA binding sequences, revealed no obvious DNA binding consensus sequence, but preferential binding to oligopurine:pyrimidine-rich stretches. Our results suggest that the observed preferential DNA binding may occur through the Zinc Finger or in a charge-charge interaction through the Ring Finger, thereby implying potentially different mechanisms for DNA and RNA MDM2 binding.

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Copy and paste a formatted citation
Spandidos Publications style
Challen C, Anderson JJ, Chrzanowska‑Lightowlers ZM, Lightowlers RN and Lunec J: Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA. Int J Oncol 40: 851-859, 2012.
APA
Challen, C., Anderson, J.J., Chrzanowska‑Lightowlers, Z.M., Lightowlers, R.N., & Lunec, J. (2012). Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA. International Journal of Oncology, 40, 851-859. https://doi.org/10.3892/ijo.2011.1267
MLA
Challen, C., Anderson, J. J., Chrzanowska‑Lightowlers, Z. M., Lightowlers, R. N., Lunec, J."Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA". International Journal of Oncology 40.3 (2012): 851-859.
Chicago
Challen, C., Anderson, J. J., Chrzanowska‑Lightowlers, Z. M., Lightowlers, R. N., Lunec, J."Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA". International Journal of Oncology 40, no. 3 (2012): 851-859. https://doi.org/10.3892/ijo.2011.1267
Copy and paste a formatted citation
x
Spandidos Publications style
Challen C, Anderson JJ, Chrzanowska‑Lightowlers ZM, Lightowlers RN and Lunec J: Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA. Int J Oncol 40: 851-859, 2012.
APA
Challen, C., Anderson, J.J., Chrzanowska‑Lightowlers, Z.M., Lightowlers, R.N., & Lunec, J. (2012). Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA. International Journal of Oncology, 40, 851-859. https://doi.org/10.3892/ijo.2011.1267
MLA
Challen, C., Anderson, J. J., Chrzanowska‑Lightowlers, Z. M., Lightowlers, R. N., Lunec, J."Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA". International Journal of Oncology 40.3 (2012): 851-859.
Chicago
Challen, C., Anderson, J. J., Chrzanowska‑Lightowlers, Z. M., Lightowlers, R. N., Lunec, J."Recombinant human MDM2 oncoprotein shows sequence composition selectivity for binding to both RNA and DNA". International Journal of Oncology 40, no. 3 (2012): 851-859. https://doi.org/10.3892/ijo.2011.1267
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