Open Access

Hydroxyflutamide enhances cellular sensitivity to 5-fluorouracil by suppressing thymidylate synthase expression in bicalutamide-resistant human prostate cancer cells

  • Authors:
    • Rumi Kawabata
    • Shinji Oie
    • Toshinori Oka
    • Masayuki Takahashi
    • Hiroomi Kanayama
    • Kohji Itoh
  • View Affiliations

  • Published online on: January 17, 2011     https://doi.org/10.3892/ijo.2011.909
  • Pages: 665-676
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Abstract

We investigated the antitumor effects of combination therapy with anti-androgens and 5-fluorouracil (5-FU), and examined the underlying mechanism of the treatment. Initially, we established the bicalutamide-resistant subline CDX25R from the androgen receptor (AR)-positive human prostate cancer cell line LNCaP through continuous exposure to bicalutamide. CDX25R cells lost the ability to respond to androgens, but still expressed AR. They showed significant resistance to bicalutamide, but had high sensitivity to hydroxyflutamide (OH-flutamide) compared with LNCaP cells. The CDX25R subline was thus considered to be a suitable model for prostate cancer that has developed resistance to first-line hormonal therapy but shows sensitivity to an alternative approach. Combined treatment with 5-FU and OH-flutamide had a synergistic effect on CDX25R cells. OH-flutamide decreased expression of the transcription factor E2F1, and subsequently of thymidylate synthase (TS), in CDX25R cells but not in AR-negative DU145 cells. This suggested that OH-flutamide enhanced the growth-inhibitory activity of 5-FU in CDX25R cells by reducing TS expression through the AR pathway. Combined therapy with 5-FU and OH-flutamide may, therefore, be appropriate for patients with prostate cancer that has acquired resistance to initial hormone therapy including bicalutamide.

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March 2011
Volume 38 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kawabata, R., Oie, S., Oka, T., Takahashi, M., Kanayama, H., & Itoh, K. (2011). Hydroxyflutamide enhances cellular sensitivity to 5-fluorouracil by suppressing thymidylate synthase expression in bicalutamide-resistant human prostate cancer cells. International Journal of Oncology, 38, 665-676. https://doi.org/10.3892/ijo.2011.909
MLA
Kawabata, R., Oie, S., Oka, T., Takahashi, M., Kanayama, H., Itoh, K."Hydroxyflutamide enhances cellular sensitivity to 5-fluorouracil by suppressing thymidylate synthase expression in bicalutamide-resistant human prostate cancer cells". International Journal of Oncology 38.3 (2011): 665-676.
Chicago
Kawabata, R., Oie, S., Oka, T., Takahashi, M., Kanayama, H., Itoh, K."Hydroxyflutamide enhances cellular sensitivity to 5-fluorouracil by suppressing thymidylate synthase expression in bicalutamide-resistant human prostate cancer cells". International Journal of Oncology 38, no. 3 (2011): 665-676. https://doi.org/10.3892/ijo.2011.909