p120-catenin isoform 3 regulates subcellular localization of Kaiso and promotes invasion in lung cancer cells via a phosphorylation-dependent mechanism

  • Authors:
    • Peng-Xin Zhang
    • Yan Wang
    • Yang Liu
    • Gui-Yang Jiang
    • Qing-Chang Li
    • En-Hua Wang
  • View Affiliations

  • Published online on: April 4, 2011     https://doi.org/10.3892/ijo.2011.995
  • Pages: 1625-1635
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Abstract

p120-catenin regulates E-cadherin stability at the plasma membrane as well as Rho GTPase activity in the cyto­plasm, and also interacts with the transcriptional repressor, Kaiso, in the nucleus. However, the role of different isoforms and the phosphorylated state of p120-catenin in the nucleus is poorly understood. In the present study, we show that p120-catenin isoform 3 interacts with Kaiso in lung cancer cells by immunoprecipitation. Nuclear-cytoplasmic extraction and immunofluoresence confirmed that Kaiso shuttled out of the nucleus via p120-catenin isoform 3. The cytoplasmic enrichment of Kaiso by p120-catenin isoform 3 was abolished due to the inhibition of chromosomal region maintenance-dependent nuclear export via leptomycin. The lung tumor tissue and cell lines expressed higher levels of the serine 288 phosphorylated form. Also, serine 288 phosphorylation in p120-catenin isoform 3 enhanced the binding with Kaiso. Moreover, immuno­fluoresence and transwell invasion assay showed that the phosphorylation of serine and threonine sites in p120-catenin induced F-actin remodelling and promoted the invasion of lung cancer cells. Collectively, our data establish that p120-catenin isoform 3 regulates the nuclear export of Kaiso and promotes invasion in lung cancer cells via a phosphorylation-dependent mechanism. Serine 288 phosphorylation can contribute to lung cancer progression.

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June 2011
Volume 38 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhang P, Wang Y, Liu Y, Jiang G, Li Q and Wang E: p120-catenin isoform 3 regulates subcellular localization of Kaiso and promotes invasion in lung cancer cells via a phosphorylation-dependent mechanism. Int J Oncol 38: 1625-1635, 2011.
APA
Zhang, P., Wang, Y., Liu, Y., Jiang, G., Li, Q., & Wang, E. (2011). p120-catenin isoform 3 regulates subcellular localization of Kaiso and promotes invasion in lung cancer cells via a phosphorylation-dependent mechanism. International Journal of Oncology, 38, 1625-1635. https://doi.org/10.3892/ijo.2011.995
MLA
Zhang, P., Wang, Y., Liu, Y., Jiang, G., Li, Q., Wang, E."p120-catenin isoform 3 regulates subcellular localization of Kaiso and promotes invasion in lung cancer cells via a phosphorylation-dependent mechanism". International Journal of Oncology 38.6 (2011): 1625-1635.
Chicago
Zhang, P., Wang, Y., Liu, Y., Jiang, G., Li, Q., Wang, E."p120-catenin isoform 3 regulates subcellular localization of Kaiso and promotes invasion in lung cancer cells via a phosphorylation-dependent mechanism". International Journal of Oncology 38, no. 6 (2011): 1625-1635. https://doi.org/10.3892/ijo.2011.995