Upregulated expression of ADAM12 is associated with progression of oral squamous cell carcinoma

  • Authors:
    • Erika Uehara
    • Masashi Shiiba
    • Keiji Shinozuka
    • Kengo Saito
    • Yukinao Kouzu
    • Hirofumi Koike
    • Atsushi Kasamatsu
    • Yosuke Sakamoto
    • Katsunori Ogawara
    • Katsuhiro Uzawa
    • Hideki Tanzawa
  • View Affiliations

  • Published online on: January 20, 2012     https://doi.org/10.3892/ijo.2012.1339
  • Pages: 1414-1422
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Abstract

ADAMs are a disintegrin and metalloproteinase family of membrane-associated metalloproteinases characterized by their multidomain structure, and have been reported to be associated with various malignant tumors. The aim of this study was to identify crucial members of the ADAM family in oral squamous cell carcinoma (OSCC), and to reveal their biological function and clinical significance. To clarify whether ADAM family genes are involved in OSCC, changes in the expression profile were investigated by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis and immunohistochemical analysis. Functional analysis was performed by comparing cellular proliferation of siADAM-transfected cell lines and parental cell lines. Real-time qRT-PCR analysis identified significantly upregulated expression of ADAM12 in OSCC-derived cell lines. This was validated in OSCC samples using real-time qRT-PCR and immuno-histochemical staining. ADAM12 expression was correlated with TNM classification; significantly greater expression of ADAM12 was observed in tumors with higher T classification and more advanced stages. Moreover, siADAM12-transfected cells showed both a suppressed proliferation rate and increased transforming growth factor (TGF)-β3 expression. Our data indicate that ADAM12 is overexpressed in OSCC and might accelerate cellular proliferation. Its function may be associated with TGF-β signaling. This study suggests that controlling the expression or activity of ADAM12 could be a useful strategy in the development of an effective cure for OSCC.

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May 2012
Volume 40 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Uehara, E., Shiiba, M., Shinozuka, K., Saito, K., Kouzu, Y., Koike, H. ... Tanzawa, H. (2012). Upregulated expression of ADAM12 is associated with progression of oral squamous cell carcinoma. International Journal of Oncology, 40, 1414-1422. https://doi.org/10.3892/ijo.2012.1339
MLA
Uehara, E., Shiiba, M., Shinozuka, K., Saito, K., Kouzu, Y., Koike, H., Kasamatsu, A., Sakamoto, Y., Ogawara, K., Uzawa, K., Tanzawa, H."Upregulated expression of ADAM12 is associated with progression of oral squamous cell carcinoma". International Journal of Oncology 40.5 (2012): 1414-1422.
Chicago
Uehara, E., Shiiba, M., Shinozuka, K., Saito, K., Kouzu, Y., Koike, H., Kasamatsu, A., Sakamoto, Y., Ogawara, K., Uzawa, K., Tanzawa, H."Upregulated expression of ADAM12 is associated with progression of oral squamous cell carcinoma". International Journal of Oncology 40, no. 5 (2012): 1414-1422. https://doi.org/10.3892/ijo.2012.1339