miR-10b promotes cell invasion through RhoC-AKT signaling pathway by targeting HOXD10 in gastric cancer

  • Authors:
    • Zhuo Liu
    • Jiaming Zhu
    • Hong Cao
    • Hui Ren
    • Xuedong Fang
  • View Affiliations

  • Published online on: January 24, 2012     https://doi.org/10.3892/ijo.2012.1342
  • Pages: 1553-1560
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Abstract

MicroRNAs play critical roles in tumorigenesis as either oncogenes or tumor suppressors. As a microRNA induced by Twist, miR-10b function as a metastasis driver in different types of cancer, in which the downstream target gene HOXD10 is the main mediator. In gastric tumor species, miR-10b levels were dramatically elevated in lymphoma node metastasis-positive tumor tissues compared with lymphoma node metastasis-free tumor tissues, and were correlated to dowregulation of HOXD10 expression. In gastric cell lines with distinct degrees of differentiation, miR-10b was highly expressed in the cell line with strong metastatic ability. In MNK45 cells, inhibition of miR-10b led to abrogation of cell invasion. While in GES-1 cells, miR-10 overexpression resulted in enhancement of invasiveness through translational inhibition of HOXD10, and constitutive expression of HOXD10 reversed the effects of miR-10b on cell invasion. Furthermore, either knockdown of RhoC or inhibition of AKT activation interfered miR-10-induced invasiveness in GES-1 cells. In summary, these observations suggest that miR-10b can stimulate the upregulation of RhoC and AKT phosphorylation through targeting HOXD10, thus promoting cell invasion in gastric tumors.

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May 2012
Volume 40 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Liu Z, Zhu J, Cao H, Ren H and Fang X: miR-10b promotes cell invasion through RhoC-AKT signaling pathway by targeting HOXD10 in gastric cancer. Int J Oncol 40: 1553-1560, 2012
APA
Liu, Z., Zhu, J., Cao, H., Ren, H., & Fang, X. (2012). miR-10b promotes cell invasion through RhoC-AKT signaling pathway by targeting HOXD10 in gastric cancer. International Journal of Oncology, 40, 1553-1560. https://doi.org/10.3892/ijo.2012.1342
MLA
Liu, Z., Zhu, J., Cao, H., Ren, H., Fang, X."miR-10b promotes cell invasion through RhoC-AKT signaling pathway by targeting HOXD10 in gastric cancer". International Journal of Oncology 40.5 (2012): 1553-1560.
Chicago
Liu, Z., Zhu, J., Cao, H., Ren, H., Fang, X."miR-10b promotes cell invasion through RhoC-AKT signaling pathway by targeting HOXD10 in gastric cancer". International Journal of Oncology 40, no. 5 (2012): 1553-1560. https://doi.org/10.3892/ijo.2012.1342