Identification of genes potentially involved in bone metastasis by genome-wide gene expression profile analysis of non-small cell lung cancer in mice

  • Authors:
    • Le Tan Dat
    • Taisuke Matsuo
    • Tetsuro Yoshimaru
    • Soji Kakiuchi
    • Hisatsugu Goto
    • Masaki Hanibuchi
    • Takuya Kuramoto
    • Yasuhiko Nishioka
    • Saburo Sone
    • Toyomasa Katagiri
  • View Affiliations

  • Published online on: January 27, 2012     https://doi.org/10.3892/ijo.2012.1348
  • Pages: 1455-1469
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Abstract

Lung cancer is commonly associated with multi-organ metastasis, and the bone is a frequent metastatic site for lung cancer. However, the molecular mechanism of organ-specific metastasis remains poorly understood. To elucidate this issue, we analyzed in this study genome-wide gene expression profiles of 15 metastatic lesions from three organs (bone, lung and liver) in a mouse model with multi-organ metastasis properties of human non-small cell lung cancer cells (ACC-LC319/bone2), using a combination of laser-microbeam microdissection and DNA microarrays. We identified 299 genes that could potentially be involved in the organ-selective nature of lung cancer metastasis. Among them, 77 were bone-specifically expressed elements, including genes involved in cell adhesion, cytoskeleton/cell motility, extracellular matrix remodeling and cell-cell signaling as well as genes already known to be involved in the bone metastasis of breast cancers. Quantitative RT-PCR confirmed the specific upregulation of eight genes in bone metastasis tumors, suggesting that these genes may be involved in bone metastasis. Our findings should be helpful for a better understanding of the molecular aspects of the metastatic process in different organs, and could lead to molecular target-based anticancer drugs and prevention of metastasis, especially bone metastasis.

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May 2012
Volume 40 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Dat, L.T., Matsuo, T., Yoshimaru, T., Kakiuchi, S., Goto, H., Hanibuchi, M. ... Katagiri, T. (2012). Identification of genes potentially involved in bone metastasis by genome-wide gene expression profile analysis of non-small cell lung cancer in mice. International Journal of Oncology, 40, 1455-1469. https://doi.org/10.3892/ijo.2012.1348
MLA
Dat, L. T., Matsuo, T., Yoshimaru, T., Kakiuchi, S., Goto, H., Hanibuchi, M., Kuramoto, T., Nishioka, Y., Sone, S., Katagiri, T."Identification of genes potentially involved in bone metastasis by genome-wide gene expression profile analysis of non-small cell lung cancer in mice". International Journal of Oncology 40.5 (2012): 1455-1469.
Chicago
Dat, L. T., Matsuo, T., Yoshimaru, T., Kakiuchi, S., Goto, H., Hanibuchi, M., Kuramoto, T., Nishioka, Y., Sone, S., Katagiri, T."Identification of genes potentially involved in bone metastasis by genome-wide gene expression profile analysis of non-small cell lung cancer in mice". International Journal of Oncology 40, no. 5 (2012): 1455-1469. https://doi.org/10.3892/ijo.2012.1348