Open Access

Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma

  • Authors:
    • Takashi Kinoshita
    • Nijiro Nohata
    • Haruko Watanabe-Takano
    • Hirofumi Yoshino
    • Hideo Hidaka
    • Lisa Fujimura
    • Miki Fuse
    • Takeshi Yamasaki
    • Hideki Enokida
    • Masayuki  Nakagawa
    • Toyoyuki Hanazawa
    • Yoshitaka Okamoto
    • Naohiko Seki
  • View Affiliations

  • Published online on: February 29, 2012     https://doi.org/10.3892/ijo.2012.1390
  • Pages: 1770-1778
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Abstract

Our expression signatures of human cancers including head and neck squamous cell carcinoma (HNSCC) demonstrated that downregulation of microRNA-133a (miR-133a) were frequently observed in cancer cells. The restoration of miR-133a in cancer cells revealed that it functions as a tumor suppressor. In this study, we investigated the novel molecular targets of miR-133a in HNSCC cancer cells and its oncogenic function, especially as it contributes to cancer cell migration and invasion. The genome-wide gene expression analysis and bioinformatics study showed that actin-related protein 2/3 complex subunit 5 (ARPC5) is a candidate target of miR-133a. Furthermore, luciferase reporter assay demonstrated that ARPC5 is directly regulated by miR-133a. Silencing of ARPC5 revealed significant inhibition of cell migration and invasion in HNSCC cell lines, SAS, HSC3 and IMC-3. In HSC3 cells, restoration of miR-133a or silencing ARPC5 led to a reorganization of the actin cytoskeleton and a subsequent change in cell morphology to a round, bleb-like shape. The expression levels of ARPC5 were significantly higher in HNSCC tissues than in non-cancer tissues. Immunohistochemistry showed that the levels of ARPC5 expression were significantly higher in invasive cancer cells. ARPC5 contributed to cancer cell migration and invasion in HNSCC and this gene was directly regulated by miR-133a. Our analysis of novel tumor-suppressive miR‑133a-mediated cancer pathways provides new insights into the potential mechanisms of HNSCC oncogenesis.

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June 2012
Volume 40 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kinoshita T, Nohata N, Watanabe-Takano H, Yoshino H, Hidaka H, Fujimura L, Fuse M, Yamasaki T, Enokida H, Nakagawa M, Nakagawa M, et al: Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma. Int J Oncol 40: 1770-1778, 2012.
APA
Kinoshita, T., Nohata, N., Watanabe-Takano, H., Yoshino, H., Hidaka, H., Fujimura, L. ... Seki, N. (2012). Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma. International Journal of Oncology, 40, 1770-1778. https://doi.org/10.3892/ijo.2012.1390
MLA
Kinoshita, T., Nohata, N., Watanabe-Takano, H., Yoshino, H., Hidaka, H., Fujimura, L., Fuse, M., Yamasaki, T., Enokida, H., Nakagawa, M., Hanazawa, T., Okamoto, Y., Seki, N."Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma". International Journal of Oncology 40.6 (2012): 1770-1778.
Chicago
Kinoshita, T., Nohata, N., Watanabe-Takano, H., Yoshino, H., Hidaka, H., Fujimura, L., Fuse, M., Yamasaki, T., Enokida, H., Nakagawa, M., Hanazawa, T., Okamoto, Y., Seki, N."Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma". International Journal of Oncology 40, no. 6 (2012): 1770-1778. https://doi.org/10.3892/ijo.2012.1390