Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer

Yamasaki,Takeshi; Yoshino,Hirofumi; Enokida,Hideki; Hidaka,Hideo; Chiyomaru,Takeshi; Nohata,Nijiro; Kinoshita,Takashi; Fuse,Miki; Seki,Naohiko; Nakagawa,Masayuki

doi:10.3892/ijo.2012.1391

June 2012 Volume 40 Issue 6

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June 2012 Volume 40 Issue 6

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Article

Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer

Authors:
- Takeshi Yamasaki
- Hirofumi Yoshino
- Hideki Enokida
- Hideo Hidaka
- Takeshi Chiyomaru
- Nijiro Nohata
- Takashi Kinoshita
- Miki Fuse
- Naohiko Seki
- Masayuki Nakagawa
View Affiliations / Copyright

Affiliations: Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan, Department of Functional Genomics, Graduate School of Medicine, Chiba University, Chiba, Japan
Pages: 1821-1830
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Published online on: February 29, 2012

https://doi.org/10.3892/ijo.2012.1391
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Abstract

Our expression signatures of human cancer including bladder cancer (BC) revealed that the expression of microRNA-1 (miR-1) and microRNA-133a (miR-133a) is significantly reduced in cancer cells. In the human genome, miR-1 and miR-133a are located on the same chromosomal region (miR-1-2 and miR-133a-1 on 18q11.2, and miR-1-1 and miR-133a-2 on 20q13.33) called cluster. In this study, we identified the novel molecular targets commonly regulated by miR-1 and miR-133a in BC. Genome-wide molecular target search and luciferase reporter assays showed that prothymosin-α (PTMA) and purine nucleoside phosphorylase (PNP) are directly regulated by miR-1 and miR-133a. Silencing of these two genes significantly inhibited cell proliferation and invasion, and increased apoptosis in BC cells. Immunohistochemistry showed that PTMA expression levels were significantly higher in BC compared to normal bladder epitheliums. PTMA and PNP were identified as new target genes regulated by the miR-1 and miR-133a cluster in BC. These genes may function as oncogenes contributing to cell proliferation and invasion in BC. Tumor suppressive miR-1 and miR-133a-mediated novel molecular targets may provide new insights into the potential mechanisms of BC oncogenesis.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer

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Abstract

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