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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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January 2013 Volume 42 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

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An International Open Access Journal Devoted to General Medicine.

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Article

Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo

  • Authors:
    • Yan Yin
    • Xu Chen
    • Chang‑Dong Zhang
    • Pei‑Feng Liu
    • You‑Rong Duan
    • Yan‑Rong Fan
    • Zhi‑Wei Wu
    • Geng‑Feng Fu
    • Jian‑Jun Wang
    • Gen‑Xing Xu

  • View Affiliations / Copyright

    Affiliations: Department of Biological Science and Technology and State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, P.R. China, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, P.R. China, School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, P.R. China, Center for Public Health Research, Medical School and State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing 210093, P.R. China, Jiangsu Research Center for Gene Pharmaceutical Engineering and Technology, Suzhou 215128, P.R. China
  • Pages: 253-260
    |
    Published online on: November 7, 2012
       https://doi.org/10.3892/ijo.2012.1691
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Abstract

Small interfering RNAs (siRNAs) are valuable reagents for efficient gene silencing in a sequence‑specific manner via the RNA interference (RNAi) pathway. The current synthetic siRNA structure consists of symmetrical duplexes of 19‑21 base pairs (bp) with 2 nucleotide (nt) 3' overhangs. In this study, we report that an asymmetric siRNA (asiRNA) consisting of 17 bp duplex region (17 bp asiRNA) exhibited potent activity in inhibiting bcl-2 gene expression and cancer cell proliferation in vitro. Importantly, this asiRNA structure significantly reduced off‑target silencing by the sense strand. To improve the stability of the 17 bp asiRNA, we synthesized a series of chemically modified 17 bp asiRNAs. Further experiments showed that in comparison with the 17 bp asiRNA, the 17 bp asiRNA‑M2, one of the modified 17 bp asiRNAs, exhibited a comparable gene silencing activity and an improved stability in vitro. Furthermore, the 17 bp asiRNA‑M2 with a proteolipid micelle delivery system can effectively suppress the growth of H22 and BGC 803 tumors in vivo. These results suggest that the chemically modified asiRNAs may have potential as an effective therapeutic approach for cancer gene therapy in the future.

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Copy and paste a formatted citation
Spandidos Publications style
Yin Y, Chen X, Zhang CD, Liu PF, Duan YR, Fan YR, Wu ZW, Fu GF, Wang JJ, Xu GX, Xu GX, et al: Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo. Int J Oncol 42: 253-260, 2013.
APA
Yin, Y., Chen, X., Zhang, C., Liu, P., Duan, Y., Fan, Y. ... Xu, G. (2013). Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo. International Journal of Oncology, 42, 253-260. https://doi.org/10.3892/ijo.2012.1691
MLA
Yin, Y., Chen, X., Zhang, C., Liu, P., Duan, Y., Fan, Y., Wu, Z., Fu, G., Wang, J., Xu, G."Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo". International Journal of Oncology 42.1 (2013): 253-260.
Chicago
Yin, Y., Chen, X., Zhang, C., Liu, P., Duan, Y., Fan, Y., Wu, Z., Fu, G., Wang, J., Xu, G."Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo". International Journal of Oncology 42, no. 1 (2013): 253-260. https://doi.org/10.3892/ijo.2012.1691
Copy and paste a formatted citation
x
Spandidos Publications style
Yin Y, Chen X, Zhang CD, Liu PF, Duan YR, Fan YR, Wu ZW, Fu GF, Wang JJ, Xu GX, Xu GX, et al: Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo. Int J Oncol 42: 253-260, 2013.
APA
Yin, Y., Chen, X., Zhang, C., Liu, P., Duan, Y., Fan, Y. ... Xu, G. (2013). Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo. International Journal of Oncology, 42, 253-260. https://doi.org/10.3892/ijo.2012.1691
MLA
Yin, Y., Chen, X., Zhang, C., Liu, P., Duan, Y., Fan, Y., Wu, Z., Fu, G., Wang, J., Xu, G."Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo". International Journal of Oncology 42.1 (2013): 253-260.
Chicago
Yin, Y., Chen, X., Zhang, C., Liu, P., Duan, Y., Fan, Y., Wu, Z., Fu, G., Wang, J., Xu, G."Asymmetric siRNA targeting the bcl‑2 gene inhibits the proliferation of cancer cells in vitro and in vivo". International Journal of Oncology 42, no. 1 (2013): 253-260. https://doi.org/10.3892/ijo.2012.1691
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