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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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April 2013 Volume 42 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells

  • Authors:
    • Peter Dynoodt
    • Reinhart Speeckaert
    • Olivier De Wever
    • Inès Chevolet
    • Lieve Brochez
    • Jo Lambert
    • Mireille Van Gele
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Ghent University Hospital, 9000 Ghent, Belgium, Laboratory of Experimental Cancer Research, Department of Radiotherapy and Nuclear Medicine, Ghent University Hospital, 9000 Ghent, Belgium
  • Pages: 1443-1451
    |
    Published online on: February 12, 2013
       https://doi.org/10.3892/ijo.2013.1823
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Abstract

MicroRNAs (miRNAs) are post-transcriptional modulators of gene expression which play important roles in tumorigenesis and cancer metastasis. Since they are often highly deregulated in various types of cancer, miRNAs may be effective treatment targets. miRNA profiling studies of melanoma have led to the identification of several tumor suppressor miRNAs. One of these include miR-145, although functional data proving its specific function are limited. Therefore, in this study, we examined the expression levels of miR-145 in three melanoma cell lines (BLM, FM3P and WM793). Additional gain-of-function experiments revealed that miR-145 exerts an anti-proliferative effect in the primary, non-invasive melanoma cell line, WM793, whereas cell migration and the invasive potential of metastatic melanoma cells was suppressed following transfection with miR-145 mimics. In order to investigate the mechanisms by which miR-145 exerts its invasion suppressor function, we examined the expression level of target genes [fascin homolog 1 (FSCN1), myosin‑Va (MYO5A and SOX9] and that of an indirect target (RAB27A) following the overexpression of miR-145. The results showed that SOX9, MYO5A and RAB27A were not involved in the biological effects caused by miR-145 mimics. Surprisingly, we discovered that miR-145 in melanoma, in contrast to many other tumor types, does not necessarily act via the target, FSCN1, since the downregulation of FSCN1 did not inhibit cell proliferation or migration but, on the contrary, increased cell invasion in two out of the three melanoma cell lines examined. Our in vitro data is in accordance with previously reported in vivo data describing the low expression of FSCN1 in malignant melanomas when compared to dysplastic nevi, suggesting that the expression of FSCN1 decreases as the formation and progression stage of melanoma advances. In conclusion, our data provide evidence that miR-145 is an invasion suppressor in metastatic melanoma cells. Despite the fact that it remains unclear which genes or pathways are regulated by miR-145 in melanoma, miR-145 may serve as a useful therapeutic agent in melanoma when re-expressed in situ.
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Copy and paste a formatted citation
Spandidos Publications style
Dynoodt P, Speeckaert R, De Wever O, Chevolet I, Brochez L, Lambert J and Van Gele M: miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells. Int J Oncol 42: 1443-1451, 2013.
APA
Dynoodt, P., Speeckaert, R., De Wever, O., Chevolet, I., Brochez, L., Lambert, J., & Van Gele, M. (2013). miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells. International Journal of Oncology, 42, 1443-1451. https://doi.org/10.3892/ijo.2013.1823
MLA
Dynoodt, P., Speeckaert, R., De Wever, O., Chevolet, I., Brochez, L., Lambert, J., Van Gele, M."miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells". International Journal of Oncology 42.4 (2013): 1443-1451.
Chicago
Dynoodt, P., Speeckaert, R., De Wever, O., Chevolet, I., Brochez, L., Lambert, J., Van Gele, M."miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells". International Journal of Oncology 42, no. 4 (2013): 1443-1451. https://doi.org/10.3892/ijo.2013.1823
Copy and paste a formatted citation
x
Spandidos Publications style
Dynoodt P, Speeckaert R, De Wever O, Chevolet I, Brochez L, Lambert J and Van Gele M: miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells. Int J Oncol 42: 1443-1451, 2013.
APA
Dynoodt, P., Speeckaert, R., De Wever, O., Chevolet, I., Brochez, L., Lambert, J., & Van Gele, M. (2013). miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells. International Journal of Oncology, 42, 1443-1451. https://doi.org/10.3892/ijo.2013.1823
MLA
Dynoodt, P., Speeckaert, R., De Wever, O., Chevolet, I., Brochez, L., Lambert, J., Van Gele, M."miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells". International Journal of Oncology 42.4 (2013): 1443-1451.
Chicago
Dynoodt, P., Speeckaert, R., De Wever, O., Chevolet, I., Brochez, L., Lambert, J., Van Gele, M."miR-145 overexpression suppresses the migration and invasion of metastatic melanoma cells". International Journal of Oncology 42, no. 4 (2013): 1443-1451. https://doi.org/10.3892/ijo.2013.1823
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