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Article

Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways

  • Authors:
    • Jinshun Zhao
    • Linda Bowman
    • Ruth Magaye
    • Stephen S. Leonard
    • Vincent Castranova
    • Min Ding
  • View Affiliations / Copyright

    Affiliations: Department of Preventive Medicine of the Medical School, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
  • Pages: 1349-1359
    |
    Published online on: February 15, 2013
       https://doi.org/10.3892/ijo.2013.1828
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Abstract

In this study, apoptosis and related signaling induced by WC-Co nanoparticles were investigated in JB6 cells and rat lung macrophages. Electron spin resonance (ESR) and fluorescent staining indicated that both WC-Co nanoparticles and fine particles stimulated reactive oxygen species (ROS) generation. Catalase exhibited an inhibitory effect on WC-Co nanoparticle-induced ROS as well as mitochondrial membrane permeability damage. Further study indicated that WC-Co nanoparticles elicited higher cytotoxicity and apoptotic induction than fine particles. Western blot analysis showed activation of proapoptotic factors including Fas, Fas-associated protein with death domain (FADD), caspase 3, 8 and 9, BID and BAX. In addition, both cytochrome c and apoptosis-inducing factor (AIF) were upregulated and released from mitochondria to the cytoplasm. Our findings demonstrate that, on a mass basis, WC-Co nanoparticles exhibit higher cytotoxicity and apoptotic induction than fine particles. Apoptosis induced by WC-Co nanoparticles and fine particles involves both extrinsic and intrinsic apoptosis pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Bowman L, Magaye R, Leonard SS, Castranova V and Ding M: Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways. Int J Oncol 42: 1349-1359, 2013.
APA
Zhao, J., Bowman, L., Magaye, R., Leonard, S.S., Castranova, V., & Ding, M. (2013). Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways. International Journal of Oncology, 42, 1349-1359. https://doi.org/10.3892/ijo.2013.1828
MLA
Zhao, J., Bowman, L., Magaye, R., Leonard, S. S., Castranova, V., Ding, M."Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways". International Journal of Oncology 42.4 (2013): 1349-1359.
Chicago
Zhao, J., Bowman, L., Magaye, R., Leonard, S. S., Castranova, V., Ding, M."Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways". International Journal of Oncology 42, no. 4 (2013): 1349-1359. https://doi.org/10.3892/ijo.2013.1828
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Bowman L, Magaye R, Leonard SS, Castranova V and Ding M: Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways. Int J Oncol 42: 1349-1359, 2013.
APA
Zhao, J., Bowman, L., Magaye, R., Leonard, S.S., Castranova, V., & Ding, M. (2013). Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways. International Journal of Oncology, 42, 1349-1359. https://doi.org/10.3892/ijo.2013.1828
MLA
Zhao, J., Bowman, L., Magaye, R., Leonard, S. S., Castranova, V., Ding, M."Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways". International Journal of Oncology 42.4 (2013): 1349-1359.
Chicago
Zhao, J., Bowman, L., Magaye, R., Leonard, S. S., Castranova, V., Ding, M."Apoptosis induced by tungsten carbide-cobalt nanoparticles in JB6 cells involves ROS generation through both extrinsic and intrinsic apoptosis pathways". International Journal of Oncology 42, no. 4 (2013): 1349-1359. https://doi.org/10.3892/ijo.2013.1828
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