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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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April 2013 Volume 42 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells

  • Authors:
    • Ying Bai
    • Guang-Rong Qiu
    • Fan Zhou
    • Li-Ying Gong
    • Feng Gao
    • Kai-Lai Sun
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Shenyang General Military Hospital, Shenyang, P.R. China, Department of Medical Genetics, China Medical University, Shenyang, P.R. China
  • Pages: 1317-1324
    |
    Published online on: February 19, 2013
       https://doi.org/10.3892/ijo.2013.1831
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Abstract

Dicer, a member of the RNase III family, is the key enzyme required for the biogenesis of microRNAs and small interfering RNAs. Recent evidence indicates that DICER1 expression levels vary among different solid tumors and decreased or increased DICER1 expression has been associated with aggressive cancers. In this study, we assessed DICER1 expression levels in acute myeloid leukemia (AML) and investigated its biological effects and transcriptional regulation in leukemia cell lines. We demonstrated that DICER1 was overexpressed in AML patients and leukemia cell lines by real-time quantitative PCR and western blot analysis. A functional assay demonstrated that the silencing of DICER1 inhibited cell proliferation and promoted apoptosis in leukemia cell lines. We also demonstrated that DICER1 was upregulated by the hematopoietic transcription factor, GATA1, through luciferase, electrophoretic mobility shift and chromatin immunoprecipitation assays. These data suggest that DICER1 plays an important role in AML and the finding that the upregulation of DICER1 is induced by GATA1 may provide a framework for the understanding of differential DICER1 expression levels in multiple types of cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Bai Y, Qiu G, Zhou F, Gong L, Gao F and Sun K: Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells . Int J Oncol 42: 1317-1324, 2013.
APA
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., & Sun, K. (2013). Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells . International Journal of Oncology, 42, 1317-1324. https://doi.org/10.3892/ijo.2013.1831
MLA
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., Sun, K."Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells ". International Journal of Oncology 42.4 (2013): 1317-1324.
Chicago
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., Sun, K."Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells ". International Journal of Oncology 42, no. 4 (2013): 1317-1324. https://doi.org/10.3892/ijo.2013.1831
Copy and paste a formatted citation
x
Spandidos Publications style
Bai Y, Qiu G, Zhou F, Gong L, Gao F and Sun K: Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells . Int J Oncol 42: 1317-1324, 2013.
APA
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., & Sun, K. (2013). Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells . International Journal of Oncology, 42, 1317-1324. https://doi.org/10.3892/ijo.2013.1831
MLA
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., Sun, K."Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells ". International Journal of Oncology 42.4 (2013): 1317-1324.
Chicago
Bai, Y., Qiu, G., Zhou, F., Gong, L., Gao, F., Sun, K."Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells ". International Journal of Oncology 42, no. 4 (2013): 1317-1324. https://doi.org/10.3892/ijo.2013.1831
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