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Article

The expression of integrins is decreased in colon cancer cells treated with polysaccharide K

  • Authors:
    • Keiko Aso
    • Takanori Goi
    • Toshiyuki Nakazawa
    • Youhei Kimura
    • Yasuo Hirono
    • Kanji Katayama
    • Akio Yamaguchi
  • View Affiliations / Copyright

    Affiliations: First Department of Surgery, University of Fukui, Yoshida-gun, Fukui, Japan
  • Pages: 1175-1180
    |
    Published online on: February 19, 2013
       https://doi.org/10.3892/ijo.2013.1832
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Abstract

Polysaccharide K (PSK), a protein-bound polysaccharide used as a non-specific immunotherapeutic agent, is said to improve the prognosis of malignant tumors such as colon cancer, but there have been few in‑depth investigations of its mechanism of action. In the present study, we investigated which genes in colon cancer cells themselves are regulated by PSK and what sort of action it exerts. Colon cancer cells were exposed to PSK in vitro and changes to their morphology and adhesive capacity were investigated, as were the changes in integrin expression that occurred. Exposure to PSK caused colon cancer cells to become spherical in morphology and investigation of their adhesion rate showed that it reduced adhesion to laminin, fibrinogen, collagen IV, collagen I and fibronectin. In terms of changes to molecules on the surface of cancer cells, there was reduced expression of integrin mRNA α and β, which are ligands for the proteins of basement membrane. An investigation of the adhesiveness of PSK-stimulated colon cancer cells to vascular endothelial cells also showed that the adhesion rate decreased significantly compared with cells not exposed to PSK. Suppression of integrin expression on the cell surface and reduced adhesion to vascular endothelial cells were observed as a novel mechanism of action of PSK on colon cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Aso K, Goi T, Nakazawa T, Kimura Y, Hirono Y, Katayama K and Yamaguchi A: The expression of integrins is decreased in colon cancer cells treated with polysaccharide K. Int J Oncol 42: 1175-1180, 2013.
APA
Aso, K., Goi, T., Nakazawa, T., Kimura, Y., Hirono, Y., Katayama, K., & Yamaguchi, A. (2013). The expression of integrins is decreased in colon cancer cells treated with polysaccharide K. International Journal of Oncology, 42, 1175-1180. https://doi.org/10.3892/ijo.2013.1832
MLA
Aso, K., Goi, T., Nakazawa, T., Kimura, Y., Hirono, Y., Katayama, K., Yamaguchi, A."The expression of integrins is decreased in colon cancer cells treated with polysaccharide K". International Journal of Oncology 42.4 (2013): 1175-1180.
Chicago
Aso, K., Goi, T., Nakazawa, T., Kimura, Y., Hirono, Y., Katayama, K., Yamaguchi, A."The expression of integrins is decreased in colon cancer cells treated with polysaccharide K". International Journal of Oncology 42, no. 4 (2013): 1175-1180. https://doi.org/10.3892/ijo.2013.1832
Copy and paste a formatted citation
x
Spandidos Publications style
Aso K, Goi T, Nakazawa T, Kimura Y, Hirono Y, Katayama K and Yamaguchi A: The expression of integrins is decreased in colon cancer cells treated with polysaccharide K. Int J Oncol 42: 1175-1180, 2013.
APA
Aso, K., Goi, T., Nakazawa, T., Kimura, Y., Hirono, Y., Katayama, K., & Yamaguchi, A. (2013). The expression of integrins is decreased in colon cancer cells treated with polysaccharide K. International Journal of Oncology, 42, 1175-1180. https://doi.org/10.3892/ijo.2013.1832
MLA
Aso, K., Goi, T., Nakazawa, T., Kimura, Y., Hirono, Y., Katayama, K., Yamaguchi, A."The expression of integrins is decreased in colon cancer cells treated with polysaccharide K". International Journal of Oncology 42.4 (2013): 1175-1180.
Chicago
Aso, K., Goi, T., Nakazawa, T., Kimura, Y., Hirono, Y., Katayama, K., Yamaguchi, A."The expression of integrins is decreased in colon cancer cells treated with polysaccharide K". International Journal of Oncology 42, no. 4 (2013): 1175-1180. https://doi.org/10.3892/ijo.2013.1832
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